[Histonet] time for penetration of methacarn fixative

Does any one have data for the time it takes for methacarn fixative (60% methanol, 30% chloroform, 10% glacial acetic acid) to penetrate and then fully fix tissue? PW Noyce -PhD student ___ Histonet mailing list Histonet@lists.utsouthwestern.edu htt

[Histonet] RNA preservation in Farmers solution

How long can tissue be stored in Farmers Solution (ethanol + acetic acid) before the RNA degrades. PW Noyce PhD student ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Toluidine blue stain fo plant tissue

Could anyone give me the concentration (? 0.1-0.05%) and the exact technique/protocol to use. I am staining shoot apical meristems not leaf or root. Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] plant tissue stain

This may seem a bit odd for this forum but what is the best stain for plant tissue histology slides-to show nuclei, cytoplasm, cell wall. PW Noyce. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listi

[Histonet] tissue fixation-formaldehyde concentrations which is best.

or discuss paraformaldehyde (which is non polymerized formaldehyde with no methanol, in water). Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] plant shoot apical meristem preparation for flow cytometry AND confocal scanning laser microscopy

We wish to measure amount of DNA in grapevine bud shoot apical meristem (small 1 by 1mm)-any advice or modern references as to fixation, permeabilization, best stain (proabably propridium iodide). Regards Peter Noyce. ___ Histonet mailing list Histonet

[Histonet] squash mounts for mitotic index count

Could anyone give me some good references as to how to best prepare this mount. Peter Noyce PhD student ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] formaldehyde concentration

( say 8% which will end up as 4% when diluted with equal amounts of glut.). Please let me know what you think. Regards Peter -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Wednesday, 31 December 2014 12:51 AM To: 'Peter Noyce' Subject: RE:

[Histonet] formaldehyde concentration

I am using pure formaldehyde with no methanol and unbuffered as a first fixative. It comes in concentrations from 20% to 4%. From my reading; 1)it seems the higher strength penetrates faster ?. 2)4% is the minimum strength to get good penetration ?. Peter Noyce PhD student Australia