RE: [Histonet] Humidity levels and IHC staining

Jason, We've experienced similar problems a few summers ago when the environmental conditions inside the Autostainers (dako) reach high temperatures and low humidity. We keep thermometers inside the stainers to monitor these levels. To remedy this problem, before every IHC run, I add a few d

RE: [Histonet] Dako's Ki67 MIB1 antibody

Diane, Looks like it did change. Lot# 4924: 80 mg/L Lot# 00065742: 35 mg/L From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of diane.cr...@amcny.org [diane.cr...@amcny.org] Sent: Thursday, Septembe

[Histonet] Direct Mouse IgG

Hello Histonet, Does anyone have a working protocol that stains directly for mouse IgG (fluorescent) on frozen sections? We are currently staining with a goat anti mouse IgG 555 (highly cross absorbed) and we have having a hard time deciding what is specific staining and what is no. Informati

[Histonet] Collagen Type VII

Hello Histonet, I'm checking to see if anyone has used (with success) a Collagen Type VII antibody on human tissue for IHC-FFPE sections? We've checked with several vendors and so far all we can find are Col VII antibodies that work only in frozen sections. Thanks! Ross _

RE: [Histonet] about ki-67

Hi Mehmet, We have used the Ki-67 antibody body from Santa Cruz Biotechnology (sc-15402, rabbit poly) on multiple species such as human, rat, mouse and canine. We have not had the opportunity to test its cross reactivity with bovine but it may be your best chance. -Ross -Original Messa

[Histonet] Collagen X

Hello again everyone, First of all, just wanted to say thanks to everyone who provided advice to my dual fluorescent stain question, it was all very helpful. Now, I am wondering if anyone knows of a good positive control tissue for Collagen Type X IHC staining? Thanks in advance, Ross

[Histonet] dual fluorescent staining

Hello everyone, I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled seconda

[Histonet] (no subject)

Hello everyone, Has anyone ever attempted to use the Leukocyte Alkaline Phosphatase Kit from Sigma (see link below) on FFPE tissues? If so can you confirm that it does or does not work? Thanks, Ross http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=86C|SIGM A&N5=SEARC

RE: [Histonet] IHC Staining Problem

We have developed a staining protocol for the CD79a antibody from two different vendors and both require a HIER TRIS/EDTA pH 9.0 retrieval. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent:

[Histonet] Pauly reagent

Does anyone by any chance have a standard protocol for histidine staining using Pauly Reagent? Thanks in advance -Ross ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] p53 IHC staining in mouse

Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antib