[Histonet] ELISA assays

Greetings my fellow histonetters, Does anyone out there that run ELISA assays use the interpretation results from the package insert or do you establish your own reference ranges? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market S

[Histonet] Gamma/Delta IHC staining using paraffin tissue

Hello fellow histonetters, I wanted to know if anyone out there is using a Gamma/Delta antibody for IHC staining using paraffin embedded tissue. I would like to work one up and I would like some guidance as far as the antibody is concerned and procedure. Thank you Albert Santiago, HT(ASCP) Lab

[Histonet] Indirect protocol

Hello my fellow histonetters, We are having difficulties with our Indirect immunofluorescent protocol, does anyone have a protocol they can share with me? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, P

[Histonet] Negative tissue controls for Special stains

Hello fellow Histonetters, I was wondering if anyone uses any specific type of tissue as a negative control for some or most special stains? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-66

[Histonet] Histology job opening

Good morning Histonetters, We here at the University of Pennslyvania Hospital have per diem and part time temp openings available at the Dermatopathology lab. If anyone is interested please contact Albert Santiago, Lab Manager, at 215-662-6539. Thank you Albert Santiago, HT(ASCP) Lab Manager P

[Histonet] New H&E stainer validation

Hello fellow histonetters, Does anyone have a procedure for validation of a new H&E stainer, if you do, can you please share it with me? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/lis

[Histonet] H&E stainer validation

Hello fellow histonetters, Does anyone have a procedure for validation of a new H&E stainer, if you do, can you please share it with me? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-65

[Histonet] FW: Formalin use in grossing

Hello again my fellow histonetters, I was hoping someone could give me their thoughts on this issue: We receive Derm biopsy specimens in formalin containers. We gross the specimen and place the cassettes with the tissue in it in a tray with 10% formalin. Although we have grossing tables with ex

[Histonet] fluorescent scope and slide scanner

Hello my colleagues in histoland, We are in the market for a Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market

[Histonet] RE: Histonet Digest, Vol 111, Issue 13

Hi Janine, we use cassette printers but we still write the number of pieces on one side of the cassette and any special instructions on the other side, such as embed on edge or embed on red ink, etc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-bo

[Histonet] FW: holes in tissue sections

From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice

[Histonet] GMS on toenails

From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail th

[Histonet] RE: Histonet Digest, Vol 97, Issue 6

Hi Toni, we've had that problem also. The best solution we've been able to find out is a pen called KP Marker Plus. You can find it at www.klinipath.com. I hope that helps, good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utso

[Histonet] RE: Histonet Digest, Vol 82, Issue 20

Hello my friends in histonet world, I'd like to offer my insight into the weekend/holiday temp.(refrigerator) recording issue. I use a traceable thermometer (Fisher Scientific) that is very simple to use and very reliable. It records the daily temp. and the weekend min./max. temp. on Friday we p

[Histonet] In-Situ Hybridization Regulations

Hello fellow histonetters, our lab is accredited by The TJC and I'd like to know if anyone knows whether automated in-situ hybridization staining requires any type of special joint commission regulation or does it just fall under the moderate or high complexity testing such as Immunohistochemistry

[Histonet] H&E Stainer Validations

Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office

[Histonet] (no subject)

Hello fellow histonetters, we're in the process of researching bar coding/specimen tracking system for our lab and I was wondering if anyone had any recommendation of any particular system and/or vendor that I should look into. Thank you Albert Santiago, HT(ASCP) Laboratory Manager Dermatopatholo

[Histonet] Nail Softeners

Hello Rene, we also do a lot of nails. I was hoping could fax me a copy of your protocol as well. Thank you .215-662-6539 -Fax Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6008/6539-office 215-662-6150-fax The information contained in this e-mail message i

[Histonet] job openings

Hello fellow histonetters. Our derm lab in Philadelphia (Univ.of Pennsylvania.Hosp.) is in search of a few good histotechs. If interested, please contact me for detailed information. Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6008/6539-office 215-662-6150-fax

[Histonet] (no subject)

Hello fellow histonetters, I was hoping someone can tell me how long it is required to store patient serum for indirect immunofluorescence and patient tissue frozen in OCT medium for direct immunofluorescence. Also, how long do we store the slides produced by these two procedures? Also, the source

[Histonet] Elisa testing

Hello all and Happy Valentine's Day, We're in the process of starting to do Elisa testing and I my question to anyone out there who is already doing it, did you go through anykind of proficiency testing and/or compliance preparedness for JCAHO or CAP before beginning to do Elisas? Thanks for your h

[Histonet] special stain instrument

Hello again fellow histonetters, I have an old artisan special stain/ihc stain stainer that I'm looking to replace with a newer model, but before I commit to it I was wondering if anyone else uses any other special stain instrument that I can look into and compare Thanks for your help A

[Histonet] ELISA TESTING certification/training

Hello fellow Histonetters, we're in the process of implementing Elisa testing in our lab and I was wondering if anyone knew of any type of certification courses, seminars, training, etc. that our techs can take. Thank you in advance for your help. Have a good day. Albert Santiago, HT (ASCP) La

[Histonet] new antibody validation forms accepted by either CAP or JACHO

Hello Histonetters, I'm looking to see if someone can suggest or provide any known CAP or JACHO "new antibody forms" that would be compliant for the inspection. Our director does not feel comfortable with the one we're presently using. Thank you Albert Santiago, HT (ASCP) Laboratory Supervis