Re: [Histonet] bone saw for cutting slabs

the one by Gryphon: Not too expensive & cuts bone well if you take your time. Other vendors like Mar-Med make alternative blades for this saw as well. Good luck, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Techno

[Histonet] change of email address

Hi Linda, I'm changing jobs, so I need to change my histonet email address. The new email address is: seanmcbride...@me.com. Thanks for all of your help, Sean McBride___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwest

Re: [Histonet] Alizarin Red on undecalcified bone

much success. There are a variety of stains to assist you in differentiation of various bone related structures. If you are not well versed with these techniques, there are labs, myself included, which can give you some pointers or contract to do the work for you. Best regards, Sean Sean Mc

Re: [Histonet] question about refrigeration

ieval into formaldehyde solution. Good luck. Best regards, Sean Sean McBride AFIRM Project Leader Senior Scientific Specialist Research Histology Services Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-2

Re: [Histonet] Xylene Resistant Labels

Hi Schaundra, I have used the StainerBondz labels from Brady for quite a while, and am quite pleased with them. Good luck! Sean McBride Senior Scientific Specialist Bone Tissue Engineering Center Research Histology Services Carnegie Mellon Research Institute Suite 4311 700 Technology Drive

Re: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

conventional staining over IHC for our results. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu

Re: [Histonet] Mouse bone blocks/sections, bone marrow smears

Hi Jian, I specialize in orthopedic histology, and I also live in Pittsburgh. I would be glad to discuss the details of your project and see if I can be of any assistance to you. Please contact me at your convenience. Best regards, ~Sean McBride Scientific Specialist Bone Tissue

Re: [Histonet] Undecalcified bone IHC

y out any developmental work. I am concerned more about the effects of the solvents used in hard tissue histology on the antigens/epitopes of interest. Does anyone use IHC staining on PMMA mineralized bone specimens on a regular basis? Best regards, ~Sean McBride Scientific Specialist Bo

[Histonet] Need SOP for Sanderson's for Thin Section

lasticized slides. Does anyone have a thin section SOP that they would be willing to share? Thanks! ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (

Re: [Histonet] embedding centers

Double ditto. Sakura TEC ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Sep 13, 2011, at 9

Re: [Histonet] blades

Dorothy, I put ours in a 15 mL centrifuge tube with a cap & sit it on the base of the microtome for the next use, that way, no one gets cut & the blade is able to be used to the fullest of it's potential. :-) Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engin

Re: [Histonet] Retirement

Hey Sally, I can attest to what you are saying. :-) I spent two summers as an undergraduate research scientist at the Inhalation Toxicology Research Institute in Albuquerque, and I had an absolutely wonderful experience. The rugged countryside is quite beautiful, and truly enchanting. I wo

[Histonet] Grossing Station Recommendation

Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research

Re: [Histonet] band saws

, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Mar 7, 2011, at 2:10 PM, Horn, Hazel V wrote

[Histonet] Request for Coverslip Removal SOP

anks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412

[Histonet] Correcting van Gieson over-stain

Hi folks, We have a set of slides that we stained in van Gieson's solution, and unfortunately, they turned out too dark. I tried immersion in 100% ethanol for ~2 minutes, and that helped a little, but they are still too dark. Does anyone have any experience or suggestions as to what solution

Re: [Histonet] Cassette Marking

Nita, We use HistoTec pens by Newcomer Supply ~Sean On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > ** High Priority ** > > If you HAVE to manually mark cassettes - what are you using? Cassette pens ? > Pencils ? What is the rest of the world doing? > > Anything else on the market? > > T

[Histonet] Rabbit Brain Tissue Processing Schedule

histonet who share their vast knowledge with those of us who are still learning. Best regards to all, ~Sean Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m

[Histonet] (no subject)

Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in

Re: [Histonet] decalcification

ion, if decal is required, I will decal in buffered formic acid solution and use a calcium reagent set to titrate to the decalcification endpoint. If you would like any specifics, contact me offline. Best regards, ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Re

[Histonet] Request for Owner's Manual: Leica SM2500E

Colleagues, I have a Leica SM2500E sliding sledge microtome for which I am searching for a copy of the owner's manual. If anyone has one that they could share with me, I would be most indebted. Best regards, ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie M

[Histonet] Request for Staining Advice: Rabbit Calvaria & underlying Cerebral Hemishpere

) evaluation. Does anyone have any suggestions for staining techniques that will show both bone & brain morphology in an en bloc specimen? Kind regards, ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh

[Histonet] Need Advice on Low Temp Tissue Processing

me that at lower temperatures, the processing times in each solution should be extended by a percentage, but by how much, I have not an idea. Does anyone have any experience or ideas with regards to low temperature tissue processing times? Best regards, ~Sean McBride Bone Tissue Engineer

Re: [Histonet] Cassettes and Processing and Fixation ~ Oh My!

ever formalin fixate a specimen for only 5 minutes. I prefer to error on the side of caution. ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641