[Histonet] IHC workshop

Hi, I am interested. Would you please send me more information? Also, do they have a workshop syllabus? Thank you, Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csego...@nsalabs.com STATEMENT OF C

[Histonet] Endogenous Tau (normal Tau)

Hi there, I am writing a poster on different Tau epitopes in human brain free floating sections. I have been unable to get any normal Tau and I wonder what could be the problem. Does anybody know what could be? Also, does anyone have a protocol that can share with me? Thank you! Claudia N. S

Re: [Histonet] mounting media for immunofluorescence

Hi Christina, We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml. Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934

[Histonet] Proteinase K treatment

Hello histoneters! This is my first time using the net. I hope to find here some answers to my problem. I am working with free floating sections embedded in gelatin. I need to use Proteinase K to detect alpha- synuclein aggregates in brain tissue. I tried concentrations in the range of 1 Ug/ml

[Histonet] (no subject)

Claudia N. Segovia Neurohistologist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csego...@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s)