Our new lot of Hematoxylin is too dark and especially overstains the mucins in
colon bxs and even membranes/umbilical cord.
Reducing the time from 3 minutes to 1 min helps some, but it the inappropriate
dark staining of the mucins is a real problem. We are also getting a film of
hematoxylin
Our state requires us to keep them for 4 years..
Before we write retention policies, we are required to look at CMS, State and
CAP requirements. I have these attachment charts in my retention policy (as
references). The policy states that we will retain for a minimum of
__(most ast
We too have experienced problems (uneven H&E staining) from such slides. We
were using Super-frost Plus without a problem, however, thought we could save a
few dollars with another vendor.
We noticed their charged slides worked too well in that they trapped water
under the slide after picking up
I am also interested in how others are processing bone marrow aspirates.
Currently, we let the aspirate clot on a watch glass for about 20 minutes,
gentle slide it onto filter paper, roll it around to get rid of the excess
fluid, place the clot between sponges, and process as usual.
However, I a
We have the same "weekend dilemma" with greater than 48 hour fixation for
breast tissue for Her-2. We send them to a huge reference lab that stated
exactly as Richard. They have found that 72 hours has little impact on
ER/PR/HER2 , however, we do request HER2 - FISH (instead of IHC) in these
ca
Ditto, to the previous messages my best stain powders are from the early
1900's.
For those CAP accredited lab, this is what CAP has to say about stains w/o
expiration dates (below)
What I have done to comply is, I have a policy that states that I evaluate
reagents at least annually a
This happens to us after extended use. Add about 5 drops/100ml of glacial
acetic acid to the light green working solution.
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Our 23 year old Reichert Jung 2030s, with high profile blades. They are work
horses Only one repair (the spring). Maintenance contract on a microtome?
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Curt is right, according to CAP ...
ANP.22570 QC - Antibodies Phase II
Appropriate negative controls are used.
NOTE: Negative controls must assess the presence of nonspecific staining in
patient tissue as well
as the specificity of each antibody. Results of controls must be documented,
either
This is the best kept secretFor those who have never tried this, you don't
know what you are missing in the ease of embedding and cutting.
We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100%
alcohols, leaving the last 100% pure (of course there is carry over during th
How has your laboratory adressed the new CAP question GEN.20425
Has your laboratory or your institution developed a solution to the
new CAP requirement below?
GEN.20425
Does the laboratory have a policy to ensure that all records,
s
We discussed this problem of weekend fixation of breast tissue (about 54 hrs in
10% NBF) with our Reference Lab and they reassured us that ER/PR IHC wouldn't
be a problem. However, when ordering Her2, select FISH method instead of IHC.
(Otherwise, if it is fixed for less than 48 hrs we would ord
I have to agree with Lynette. Good solution as well ...change only one thing at
a time.
We occasionally have the same problem and the BM clots are the only tissues in
the entire batch to fall off (It is usually the blood in the center of the
section, the rim stays attached, which is the clue th
Our Pathologist found it more time consuming to delete lines that were
non-applicable than to dictate what they do want from the CAP Cancer Protocols:
1. I copied and pasted the commonly used protocols into the canned text
dictionary (in meditech, magic) then each pathologist will edit it to mee
1. Most bxs we cut 2 levels (one level/slide, no more than 2 sections/slide).
2. Prostate bx are unique. Our Urologists love us and send color coded bxs in 2
containers (Left & Right, yellow = apex, green = midgland, red= transition,
blue = base). We then are able to place all bxs in a total of
Michael,
Ditto, very well stated. I too believe that our industry is under appreciated.
Many new grads of today find a two year degree demeaning and wouldn't consider
HT because of it. I don't understand how some professions like pharmacy &
physical therapy gain respect and grow to create 5 yr,
Tiffany,
I was searching Histonet to find the answer to a similar question, but you look
like the only one out there with this interest. I know it was back in 07, but
may I ask how did your interface finally work out?
We also have Meditech Magic, and I am looking for a way to have our current
re
We started staining prostate needle bxs w/ PIN 4 (triple stain).
1. My understanding is that we can bill for 88342 x 3 (per specimen) if a
comment is made on the results of the nuclear staining and cytoplasmic staining
of the DAB and the staining of the Vulcan red. The key is documentation in th
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