[Histonet] Movat Pentachrome--Saffron stain of collagen

2009-10-06 Thread Stephens, Elizabeth Humes
I’m trying to find out more information about Movat saffron-straining collagen as it relates to collagen maturation/cross-linking. Does anybody know whether more aligned or more cross-linked collagen will pick up more saffron in a Movat pentachrome stain? (We mostly are dealing with collagen

[Histonet] Fixing and IHC on PEG hydrgoels

2008-12-18 Thread Stephens, Elizabeth Humes
Dear Colleagues, I was wondering if anyone has had any experience with fixing and performing immunohistochemistry on cells seeded onto PEG hydrogels. These are very hydrophilic gels and I have heard that, even with a very short fixation time, fixation causes any cells that were growing on the P