[Histonet] SMAD4 antibody

Hi Jessica, I have worked up a SMAD4 clone B-8 on the Autostainer Link 48 feel free to reach out if you would like the protocol. Kind Regards, Kathy Kathryn Stoll, HT(ASCP) Manager, Precision Medicine Laboratory (PML) Phone: (414) 955-4573 | E-mail: kst...@mcw.edu Precision Medicine Lab custom

[Histonet] IHC on old slides

Hi Everyone, I have been doing some IHC staining on slides that are 20 years old or more. Depending on the antibody the staining is pale in comparison to my control slide. Does anyone have experience with staining older slides? Do you have any tips to share? The slide storage conditions ar

[Histonet] Brain Tissue Processing

Hello, I am looking for help with tissue processing of brain. The pieces are large, they fill a super cassette. Does anyone have a protocol they could share. Thanks in advance. Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. W

[Histonet] Microm 335E Retraction

I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226

[Histonet] TMA help

Hello Histonetters, I have a PI who made a very large TMA and it is half detatched from the base of the cassette. Does anyone have any tips on trying to stabilize it prior to cutting? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9

[Histonet] Dako CMV

Hi, I need to work up the Dako CMV for a project. I have an Autostainer Plus and an Autostainer Link 48. Would someone be willing to share their procedure with me? Many Thanks, Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W.

[Histonet] BRAF V600E

Is anyone running NewEast BRAF V600E (cat 26039) on the Dako Link48 with Envision FLEX? I am wondering what your dilution and retrieval are. I am having trouble with this antibody. Kathryn Stoll Department of Pathology Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Ph: 414-805-1

[Histonet] Sections washing off slides

I have some old H&E slides that I would like to try IHC staining on. I am having trouble keeping the tissue on the slide. I put a slide in xylene to remove the cover slip and eventually the section floated off the slide when I tried to rehydrate. The second time I tried Mount-Quick from Newcomer

[Histonet] CDC42

Does anyone have a good protocol for CDC42? The ab I am using if from santa cruz sc-8401. I stain on the dako autostainer plus using flex reagents. Kathryn Stoll, HT(ASCP) Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu _

[Histonet] reagents without expiration dates

Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? CAP checklist ANP 21382 Thanks, Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu

[Histonet] Topoisomerase I

I am using TOP1 that originally worked at 1:100 dilution. I am now using at 1:20. Any ideas why the staining has decreased so much? I have it stored in -20 in aliquots. Or has someone found a TOP1 that works for them. It is too expensive to run at 1:20. Thanks, Kathryn Stoll, HT(ASCP) Depat

[Histonet] Looking for IHC tips

Does anyone have some tips to work up antibodies on the Dako Autostainer using the FLEX detection kit. I have run into a few research antibodies that are not staining well or at all. Thanks Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwauk

[Histonet] Processing Mouse Pancreas

Happy Friday Everyone, Does anyone have a protocol for processing mouse pancreas? We have a VIP6. I am not familiar with the processing any animal tissue and do not know if it is similar to human. Thanks in advance. Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 92

[Histonet] Paraffin blocks from cell line

Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical C

[Histonet] Vendor for antibodies - research

I am switching from hospital based IHC to research in a new lab. I am hoping to get some input for which vendors have shown good results for antibodies that I have not previously used. I am looking for a few specific ENT-1 RRM-1 ERCC TYMS SPARC TOPO-1 Thanks __