[Histonet] DAKO Ubiquitin backorder

Hi histonetters, I was wondering if anyone has come across a backorder problem with DAKO's Ubiquitin antibody for IHC (polyclonal Z0485) and switched to a new clone with equivalent results? I was looking at the Cell Signaling (rabbit polyclonal) and Novocastra (monoclonal mouse FPM1). We are us

[Histonet] DAKO LINK PTs

Hi Histonetters, I have encountered a problem with some of my staining and I am struggling to pinpoint the root cause. I was wondering if any other DAKO Autostainer Link/PT users are experiencing the same oddities. It seems like the first runs with fresh HIER PT solution is very typical of wha

[Histonet] p16 [INK4] clone G175-405

Hi Histonetters, I am trying to work up the Biogenex p16 on FFPE tissue and I am not having much luck. Does anyone have any experience with this antibody and getting it to work. I have tried a wide range of dilutions and epitope retrieval (both heat and enzyme). Any suggestions? Thanks in adva

[Histonet] Bone Saw for surgical specimens

Hi Histonetters, I am looking for a bone saw for smaller surgical specimens (like jaw bone). Any suggestions or input about what is working or not working for you would be helpful. Thanks in advance! This email and/or any documents in this transmission is intended for the addressee(s) only a

[Histonet] Formalin Neutralization

Hi Histonetters, We are looking to other labs for information and suggestions for formalin neutralization. What products do you use and do they work effectively? Thanks for your input!! Tiana Baskin This email and/or any documents in this transmission is intended for the addressee(s) only and

[Histonet] RE: Biocare Decloaker Nx Gen

I have had the pleasure of seeing a demo of these Nxgen decloakers and I was very impressed. I currently use the older generation ones which still work very reliably for me. There is a nice tray inside the chamber to ensure the slide containers do not tip over and the USB which tracks all of the

[Histonet] IHC Stainer Dispense volume

I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large tissu

[Histonet] ACTH Antibody

Hi I am working with the DAKO ACTH clone 02A3 (concentrate) and I am getting a lot of background staining. I have tried to decreasing the incubation time of the primary and I have tried to increase the dilution however I am starting to loose signal and I still have the background and non-specific s