Re: [Histonet] Red chroamgen/LeicaBond andBondMax

John, yes, it is their Bond Refine Red detection kit Tim morken Sent from my iPhone > On Jun 9, 2022, at 10:23 AM, John O’Brien via Histonet > wrote: > > Can some tell me if the Leica IHC Bond or Bond Max can preform the Red > Chroamgen IHC stain

Re: [Histonet] Advice for new grads?

. Especially the vendor work which was in product development and entailed visits to many labs and other vendors, even internationally. That work really opened my eyes to both sides of the field. If something like that comes along, and fits with family/life, it is well worth doing. Tim >

Re: [Histonet] Advice for new grads?

ution works, and how you get to know people around the institution, especially outside your own lab. A lot of problem solving in the lab is knowing who can help you. You never know when something you learn will come in handy even years later. Tim Morken > On 05/09/2022 9:55 AM Pam Bark

Re: [Histonet] 10% buffered formalin storage

same ventilation system as the fume hoods. That is to prevent overwhelming fumes if one or more bottles should leak. Tim Morken Supervisor, UCSF Histology, EM and Neuromuscular Specials Studies (ret.) > On 05/06/2022 7:10 AM Richardson, Pam K via Histonet > wrote: > > > I have

[Histonet] Subject: braf (v6001e)

Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression. Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp tim.higg...@cddmedical.com Message: 6 Date: Wed

[Histonet] Subject: Re: Trouble shooting question

hanks, Tim -- Message: 6 Date: Thu, 12 Jul 2018 12:26:21 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Trouble shooting question Message-ID: Content-Type: text/plain; charset="UTF-8" My pathologists says that 1 out of 100 (his e

Re: [Histonet] Antibody Validation CLIA

Cap states, "should test a minimum of 10 positive and 10 negative tissues". Does not state "cases" but "tissues", so we used a block for positive and negative from the same case. Thanks, Tim -Original Message- From: histonet-requ...@lists.utsouthwest

Re: [Histonet] Hematoxylin Precipitate

like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is

Re: [Histonet] Hematoxylin Precipitate

they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "

Re: [Histonet] IHC negative controls

We run patient tissue as a negative and we use diluent as the reagent negative. TH > Message: 7 > Date: Fri, 17 Jul 2015 14:48:17 + > From: "Abbott, Tanya" > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] IHC negative controls > Message-ID: > > <852f7d2c14fb4

[Histonet] Allowable temperature range

What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] RE: Buffer pH monitoring

I check pH every time I make a new batch(bottle) and then periodically there after. Timothy N. Higgins, HT (ASCP), QIHC > Message: 10 > Date: Tue, 11 Feb 2014 16:23:22 + > From: "Gupta, Nilesh" > Subject: [Histonet] Buffer pH monitoring > To: "'histonet@lists.utsouthwestern.edu'" >

[Histonet] RE: Ventana LEAN

e. Thank, Tim H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Embedding paraffin for brain tissue?

but soon I experienced rolling of the sections. I have tried changing the angle of the blade, to no avail. So, I thought that I would try a change in the embedding paraffin. Thanks for any suggestions that you may have, Tim Wheelock Harvard Brain Bank McLean Hospital B

[Histonet] FW: Fungus Controls

I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does

[Histonet] Immunostaining after prolonged formalin immersion?

elves. Thanks, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] edge effect on IHC

Hey Mary, I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which use

[Histonet] Dimwits

hed to this response unless your suggesting everyone on Histonet are dimwits. Also, not sure why you are attacking the "youngsters" on this forum, this forum is for the young, old and middle-aged. Have a fantastic Friday TiminTexas not Tim M. (Don't want anyone mad at him o

[Histonet] RE: Histonet Digest, Vol 118, Issue 23

llowed to share their personal protocols, procedures, opinions, and experiences on here anymore. Come on Rene J., next time sh!! I should have just hit the "Delete" button and follow my own advise! Tim N. Higgins, HT (ASCP), QIHC > Message: 7 > Date: Thu, 12 Sep 2

[Histonet] Embedding problems-part 2

, and so forth. Possibly the heat of the extra bit of wax is causing the lifting? Thanks again Tim Wheelock ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Embedding problem

more tissue than I would like to obtain a full face for sectioning. Has anyone had this problem? How do people avoid it? Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Batch Controlling for IHC

sense, just wondering if this is something others have dealt with. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Processor dehydration cycles

solution, and so avoid strong currents from harming the cells. Also, brain tissue may be more delicate that say prostate, skin or uterus. By the way, I actually do not know whether this "rational" is correct or not. However, in general, if you can start at 70%, it can't do any harm. Ti

[Histonet] RE: High complexity test

n kits work and antibodies react so and so forth, but its still not considered high complexity. Best way is to call CAP or whatever governing body your under and ask the question and document who you spoke with if there is nothing in writing. Tim H. > > > From: Sara Bal

[Histonet] Question about sectioning brain tissue at 10-15 microns

stain) tend to curl, but I just guide them off of the disposable blade with a brush or a pick. Hope this helps, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] LFB Lithium Carbonate Differentiator alternative

staining dish with tap water. Hope this helps, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] MOHS

I need some MOHS procedure help, anyone want to help me offline? I would really appreciate it!! Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] Re: Question

All I have to go by is CAP's response to the question, "Is IHC a High Complexity Test". Their verbal answer is no, but there is nothing formally documented. The only documented statement is from 2010 Webinar during the Question and Answer portion. It is as follows, Laboratory Accreditation

[Histonet] Re: Question

The professional interpretation is considered a high complexity test but not the actual technical component. Thanks, Timothy N. Higgins, HT (ASCP), QIHC ___ Histonet mailing list Histonet@lists.utsouthw

[Histonet] Laboratory SOP

Hi Everyone: I am putting together an SOP for my neuropathology lab. Does anyone know of a template, example, or guide that I could refer to? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet

RE: [Histonet] interview

offered for the benefit of all, and especially for the benefit of those posting a problem. At least that is what I always try to do! René J. From: "joellewea...@hotmail.com" To: Tim Higgins ; histonet@lists.utsouthwestern.edu Sent: Thursday, January 10, 2013 11:07 AM Subject: Re:

[Histonet] interview

ualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks. It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 + (UT

[Histonet] Histotechnician Salary 2

Hi again all: I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. We have our immunostains done by another lab. Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet

[Histonet] Histotechnician Salary

can work independently. However, I have no idea what the salary would be for this kind of position. Could people let me know how much such a position-or a comparable one in a surgical histology lab-pays? Thank you, Tim Wheelock Assistant Director, Neuropathology Laboratory Harvard Brain Tissue

[Histonet] VIP6 Questions

manually, and if so, why? Also, do you find the touch screen graphics easy to use? Have any problems developed with the display itself? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet

[Histonet] Follow up question for embedding centers

? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] Embedding Centers

so do not feel comfortable having the cassettes sitting dry in the holding tank Thanks, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Tissue Processors

use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmon

[Histonet] RE: CRYOSTAT PROBLEM

k for the service desk. Best Regards, Tim Coulter Operations Manager | Belair Corporate Group tim.coul...@belairinc.com | p 973.912.8900 ext. 174 | f 973.232.0077 Avantik Biogroup | Belair Instrument Co. | Sierra Biosystems 36 Commerce Street | P.O. Box 619 | Springfield, NJ 07081-0619

Subject: [Histonet] Background staining on H pylori

Hey Deloris, I would have the dispensing nozzles on the XT checked, could be it is not getting rinsed properly. Had that issue with my Ultra not to long ago. No warnings or errors showed up, just a clogged nozzle tip. Tim -Original Message- From: histonet-boun

[Histonet] Re: Dako vs. Ventana IHC systems

know a thing about IHC's but can run a piece of equipment. I am not saying every Ventana user is not a skilled tech, clarify that before I get any back lash on that statement. Either one will do the job. Tim ___ Histonet mailing list His

[Histonet] Re: Cutting Blocks

me they get evaluated and receive the same evaluation as the work they have put forth. Best way for them to get the message you are trying to get across given you have been up front with them preceding the evaluation. Tough subject and everyone has a opinion on what is right. My two cents. Tim

[Histonet] blocks and slides

Hey Dorothy, It's 10 years for CAP, but you need to check with your state as well. I know for New York you must hold them for 20 years. Thanks, Tim Message: 20 Date: Tue, 11 Sep 2012 08:09:24 -0700 (PDT) From: Dorothy Glass Subject: [Histonet] blocks and slides To: his

[Histonet] Gloves

does not break. However, now that you mention it, I wonder if a single layer really totally prevents penetration of xylene or not. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 02478 ___ Histonet mailing list Histonet

[Histonet] Bielschowsky questions, Luxol Fast Blue answers, and Protocol Drift.

to switch from staining the tissue for 2 hours at 60C to a full over-night (why I never needed to switch times before is a mystery). That did the trick beautifully. The myelin is staining perfectly again. Thanks again, Tim Wheelock Harvard Brain Bank B

[Histonet] Re: Frozens and antigen retrieval

Agree, only if the antigen has been bound by a fixative. -Original From: Daniela Bodemer To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 4, 2012 7:02 AM Subject: [Histonet] Frozens and antigen retrieval Hi all, A question from my student: antige

[Histonet] Hydrometer questions

yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592

[Histonet] Luxol Fast Blue problem

. But I suspect there may be other factors involved. Has anyone had this sort of problem before? What did you find was the cause and how did you fix the problem? Thanks for any advice you can provide. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA

[Histonet] Pens for writing cassettes

. There was no fading on the tissue cassettes at all. The writing was as clear and crisp as when they were first written. I also can still use the Securline Marker 2 pens for writing out my slides. Thanks again, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA

[Histonet] Thank you

. If we still have a problem, we will experiment with a different cassette. Thanks again. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] Cassette labeling problem

erature of the embedding media is pulling the ink off. Has anyone experienced this before, and if so, how did you overcome it? Are the Securline Marker 2/Superfrost pens appropriate for cassettes? Thank you for any advice you can provide, Tim Wheelock Harvard Brain Tissue Resource Center McLea

[Histonet] Thank you

Hi All: I want to thank everyone for the advice on xylene-resistant gloves. It was really helpful. Tim Wheelock Tim Wheelock Assistant Director Harvard Brain Tissue Resource Center Instructor In Neuroanatomy 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax

[Histonet] Gloves for coverslipping?

Hi Everybody: Does anyone know of a xylene-resistant glove that can be used for coverslipping, and that allows the dexterity necessary to coverslip? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list

[Histonet] thermo excelsior es

what are the disadvantages and advantages of excelsior ES tissue processor? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Subject: [Histonet] nuclear fast red

Nuclear Fast Red needs to be heated during prep and it does take a little while to dissolve, it never really dissolves entirely, probably saturates the solution pretty fast. Tim Message: 1 Date: Tue, 29 Nov 2011 11:29:07 -0700 From: "Patsy Ruegg" Subject: [Histonet] nuclear f

[Histonet] Spirochete Control Block

Hello Histonetters, Anyone have a spirochete control block(s) they would like to trade? Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histostar Embedding Station

Hey Jessica, We have two Histostar's that have been here since March and have not had any issues with them. Can't say anything bad about them, yet. Thanks, Tim Subject: [Histonet] Histostar Embedding Station To: "Histonet@lists.utsouthwestern.edu" Message-ID: <131

[Histonet] p57 antibody

to be would ideal. Any other help would also be greatly appreciated. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] DISPOSAL OF SPECIMENS

I have been in the histology field for 20 years and never heard of having to remove the labels prior to disposal through an approved vendor. I don't believe that the labels from blood tubes are removed prior to disposal, this would be the same type of scenario. Thanks, Tim Message: 9

[Histonet] p57 kip2 (Ab3) concentrated antibody

Does anyone use the p57 (Ab3) concentrated antibody from Lab Vision (Fisher) for Molar pregnancies on the Ventana platform using the UltraView detection system? What is the protocol and dilution you run? Any help would be greatly appreciated!! Thanks, Timothy Higgins, HT(ASCP), QIHC Histolo

[Histonet] Tissue handling at embedding

I have worked at labs that reuse molds and ones that clean between uses. Guess it all depends on what your definition of a "clean" mold is. If you have the time and space, not reusing the molds is best practice but not always practical. Thanks, Tim Message: 10 Date: Tue, 20 Sep 201

[Histonet] STP420ES

Good instument, been working with it for about 6 months, using the Fast Flex system for rapid tissue processing. What did you want to know specifically? Tim Message: 8 Date: Tue, 13 Sep 2011 09:11:53 -0700 From: Davide Costanzo Subject: [Histonet] STP420 To: histonet@lists.utsouthwestern.edu

[Histonet] IHC validation

ld be appreciated. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Re:peggy wenk comments on HT/HTL practical

of peoples lives. Maybe actual training is not as big a deal if you are flipping burgers but Histology is slightly more important than that. At least I feel it is, obviously the people running some of these "Approved" programs don't think so. Scary!! Thanks, Tim - Original

[Histonet] RE: Re:peggy wenk comments on HT/HTL practical

worked so hard to receive in the healthcare world. Wow!! Thanks, Tim -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:

[Histonet] Shandon Embedding Center Advice--Thank you

again. However, as a result of this experience, I now have some back-up options for buying a new or refurbished machine or renting/leasing one in the future. I will try to put in for a new one next year for our next grant-cycle. Thanks again, Tim Tim Wheelock Assistant Director, Neuropathology

[Histonet] Embedding Center Questions

that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue

[Histonet] Job opening

GI path lab in Santa Rosa, CA looking for a certified tech. Great opportunity!!! Great pay. Flexible hours. Friendly office. Submit resumes to tja...@yahoo.com. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/

[Histonet] Re: Recycled alcohol

Eosin are you using? Thanks, Tim Message: 9 Date: Fri, 22 Jul 2011 07:06:19 -0400 From: "histot...@imagesbyhopper.com" Subject: [Histonet] Recycled alcohol To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi Histonetters! F

RE: [Histonet] Controls with patient specimen on same slide

It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim - Original Message - From: To: Sent: Tuesday, July 19, 2011 6:04 PM Subject: Histonet Digest, Vol 92, Issue 25 Hi, > I'm

[Histonet] Re: Histonet Digest, Vol 92, Issue 18

On all stomach biopsies. Tim - Original Message - From: To: Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http

[Histonet] Control Tissue

1 p16 AFB Fungus I will need is your: FedEx or UPS # Name Department Address Phone # Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Fw: [Histonet] Re: Embedding Media

sing one paraffin type is > fine, all personal preference. We could go on forever with this!! > > > I am not endorsing any paraffin on here, used many different paraffins and > each work as anticipated. > > Thanks, > > Tim > > > > > Sent: Monday, July 11,

[Histonet] Tissue controls

I have an abundance of H-pylori controls that I would be willing to part with and a number others as well. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Embedding Media

higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: "Paula L

[Histonet] 100 micron paraffin sections?

tissue in paraffin and cut 100 micron sections. Does anyone have a protocol for processing this kind of tissue into paraffin and then sectioning and mounting such thick 100 micron sections? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue

RE: [Histonet] IHC validation

. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 3:20 PM To: Weems, Joyce; Joe

RE: [Histonet] High Complexity Testing

pretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Origin

RE: [IHCRG] RE: [Histonet] batching controls

What is the purpose of batching control? Unless you are having a hard time finding certain positive tissue types, it's good practice to run a positive control with each case to ensure every slide shows the desired results. Plus the patholigist have to call and verify that the control worked, wasti

[Histonet] methylene blue stained tissue

Hello Histies, I received rodent dermis that has been stained with methylene blue. I would like to perform immunolabeling experiments on this tissue. Should I expect any interference from the methylene blue stain? Tim Quinn Office of Research Administration MEDLAB Senior Research Technician

RE: [Histonet] RE: Physician signature

Joyce wrote: "The Federal Register is always a good place to find interesting reading" Gee, Joyce, all these years I've known you and I never realized you are a masochist!! But maybe the fact you are the go-to person for regulations should have been a clue!;) Tim Morken

RE: [Histonet] EM cacodylate

st look up "Carson millonig" and you'll find several commercial sources. Or, of course, look at Carson's book for the formula. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwest

RE: [Histonet] Competencies

We keep ours for two years. We are under JC and that is how far back they go when checking. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] prostate orientation

c yield of prostatic core needle biopsies," Rogatsch, H et al, The Prostate, 42:124-129 (2000). Thanks ahead of time! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center ___ Histonet mailing list Histonet@lists.utsouthw

[Histonet] (no subject)

Hi Histonetters, This maybe a stupid question but I'm going to ask anyways. Is it legal to buy tissue blocks (with human tissue) from another lab to use as controls for specials and Immunos? I know we purchase slides from vendors to use with our specials and Immunos. Thanks!

RE: [Histonet] unstained paraffin tissue slides storage--why cold?

Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday

RE: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path

logy in-process misidentification defects by bar code-specified work process standardization. Am J Clin Pathol. 2009 Apr;131(4):468-77. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern

[Histonet] Training labs in the San Francisco Bay Area?

Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.mor...@ucsfmedctr.org<mailto:tim.mor...@ucsfmedctr.

RE: [Histonet] Formalin Fixation - HER2 testing

ffect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596." Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard C

RE: [Histonet] mislabeling of slides and blocks

Glad I never worked at that lab, never would have made to a supervisory role. I have to agree with Sarah, two infractions in a 12 month period is extremely severe. I don't care if you have Vantage or any one of the million tracking software packages out there. Especially with the shortage of qu

RE: [Histonet] problems with staining IHC

Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message--

RE: [Histonet] change in immuno machines

Agreed. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, October 01, 2010 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

RE: [Histonet] Marking inks

We use Statlab and Fisher marking inks. We dip our specimens in Bouin's or Vinegar to set the ink. We don't have any problems with the ink coming off. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:his

RE: [Histonet] Marking inks

We use Davidson inks, except for the green. The green was gumming up our VIP processors. In fact we found out that Dr. Davidson does not recommend using the green for tissues to go through a processor, but that was not in the datasheet. So we use Cancer Research for the green ink. Tim Morken

Recall: [Histonet] FW: DAB

Morken, Tim would like to recall the message, "[Histonet] FW: DAB". ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Ventana Ultraview DAB detection kits for sale.

Hello Histonetters, We are overstocked on Ultraview DAB detection kits and looking to sale some for $2,300.00 per kit. We will pay for shipping within the continental US. The kits are NOT registered with the button still attached. We have 6 with the expiration Date: 4/2011 Lot #812955 We

RE: [Histonet] RE: Questions

Agreed! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA Amarillo, TX -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, September 20, 2010 10:33 AM To: Senn, Amy

[Histonet] Ventana Ultraview Detection

Hello Histonetters, We are in the process of selling some of our Ventana Ultraview Detection kits. The kits still have a long shelf life and have not been registered. If you are interested in purchasing one of more of these kits please email me with questions and or contact info. Thanks, Tim

RE: [Histonet] ANP. 23075

them in a reader and download the info to a software program that allows you to make graphs. They are also great for logging temps when shipping items that are temperature sensitive. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message

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