[Histonet] Subject: braf (v6001e)

2018-09-19 Thread Tim Higgins via Histonet
Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression. Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp tim.higg...@cddmedical.com Message: 6 Date: Wed

[Histonet] Subject: Re: Trouble shooting question

2018-07-12 Thread Tim Higgins via Histonet
Hey Charles, I have to agree with Rene. They are either being left out after sampling prior to fixation or possibly coming into contact with air during the processing run. Make sure your solution are covering all the cassettes, don't let them float free inside the processing chamber. Thanks,

Re: [Histonet] Antibody Validation CLIA

2018-03-20 Thread Tim Higgins via Histonet
Cap states, "should test a minimum of 10 positive and 10 negative tissues". Does not state "cases" but "tissues", so we used a block for positive and negative from the same case. Thanks, Tim -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@