Re: [Histonet] Wax in hallways

We just request that our building maintenance does a quarterly deep floor cleaning in our lab. In the past I know they used a citrus type cleaner. Besides that , they just conduct their regular nightly cleaning. -Original Message- From: Naira Margaryan via Histonet Sent: Monday, July

[Histonet] New lab set up

We will be setting up our lab on the 2nd floor in the next few months and since it's been a while since I have set up a new lab I am looking for any ideas you all have. Our lab is a small one that does derm/H&E only for our 3 locations. I have always stood to section, but am thinking of getting a

[Histonet] Folded Sections

>From one day to the next I am getting folded sections after staining. There >does not seem to be an issue when sectioning. I see the folding once the slide >is stained. I only do skin, any ideas what is going on? No changes in >procedure or reagents from last week to this week. V. Avalos ADS H

[Histonet] MOHS TECH NEEDED - ARIZONA

MOHS TECH - IMMEDIATE OPENING - ARIZONA Derm office in immediate need of an experienced F/T MOHS TECH. Will work with one Mohs Surgeon and must have dependable transportation to alternate offices daily. Offices are in Surprise and Glendale, approximately 11 miles apart. If you or anyone you

[Histonet] RE: Xylene Substitute Protocols

Statlab XS-3 & Acrymount. I do H & E staining only. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 9:50 AM To: histonet@lists.utsouthwestern.edu Subj

RE: [Histonet] Eosin Leaching

We had the same problem. Increase your Alcohol/Reagents (following Eosin) times as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and rotate the 100% each day. If using a Xylene Sub it could also be a

RE: [Histonet] Shandon Grosslab Senior

Mercedes Medical sells their own brand that are compatible with others out there and sell for less than Thermo. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson Sent: Thursday, December

[Histonet] RE: Xylene Substitutes

I use XS-3 from Statlab for my H&E staining & Iso Alc for my overnight derm processing. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Wednesday, August 21, 2013 8

[Histonet] Lab Equipment Repair in AZ??

We recently found out that our equipment repair tech has retired. For our PM's and small repairs of our non contracted lab equipment we have used a local company for years. Sad to see him go. Does any one have any other local medical lab equipment techs/companies out in AZ (Phoenix) they would r

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck. V.Ava

RE: [Histonet] Xylene Substitutes

I use XS-3 from Statlab for staining. Since it is substitute I had to do some adjusting to the stain line but once I got it right I have had no problems We pay about $70 for 4 gal. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsout

[Histonet] Xylene Free Process

Hello, I have been processing our tissue (derm only) in our Shandon Excelsior using a xylene free process with Iso Alcohol. A few years back it was set up that way by a Thermo rep and it worked really well. I am now having issues and the docs seem to think it is a processing issue rather than t

RE: [Histonet] how often do you change your hematoxylin?

We are a small lab and used Vintage for a while and loved the stain. I did notice that both the Eosin and Hemat did become watered down faster than any other brand we had tried and evaporated more. I also could only use it for about 2 wks before having to toss and refill. If it was more cost ef

[Histonet] RE: xylene substitute for processing

I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for us. No problems with the processing. I also use sub xylene in staining with a few extra steps but no problems. -Original Message---

RE: [Histonet] Paraffin Blocks Cracking

I have also noticed that the temperature in the lab makes a big difference. Now that it is colder outside and some heaters kick on check your temps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brian f

RE: [Histonet] thermo excelsior es

I love our excelsior. Our previous processor was an ancient VIP. We have had it for over 5 years. Pros: -You save on chemical if you program your chemicals to rotate based on the first Alcohol quality. It really works, I have tested my alcohols to make sure they were at the correct percentages

RE: [Histonet] Slides sent out for outside consult.

Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only

FW: [Histonet] Formaldehyde monitor

We use Advanced Chemical Sensors. Acsbadge.com is website. I also used to use Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 11:29 AM To: histonet@

FW: [Histonet] CLIA classes?

I work at at derm office and this would be great to know about if there was one. Please please please include me in the list of responses. Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sands, Jenn

[Histonet] Tap Water or filtration system

We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to h

[Histonet] Pouring Chemicals in Stainer

I was curious about any tricks out there for avoiding spills when setting up the stainer. We will be receiving a new stainer soon which will be taller and larger than our current one. I'm just worried I will spill chemical into the surrounding dishes. Would using wash bottles work or are there an

RE: [Histonet] Cassette Marking

Most places will also send you a sample. This way you can run test blocks in your processor to make sure you are happy w/ the results. Just remember to label the block w/ the pen name so you wont get confused! :) I currently use Mercedes Platinum Marker -Original Message- From: histonet-b

[Histonet] Reagent dispenser

Does anyone have a method of topping off and dispensing their reagents into the Autostainer or anyother stainer. Was thinking about using wash bottles. I want to avoid as many counter spills and overflow into the next dish as I can. V.Avalos ADS, INC Fax:602-277-2134 ___

[Histonet] Autostainer Input

Our Linear stainer is at its last days and we are looking at purchasing an Autostainer. The two that I have been asked to look at are the Leica Autostainer XL & Sakura DRS 2000. Does anyone have any input on either of the two? Good & Bad will be appreciated!! V.Avalos ADS, INC Fax:602-

[Histonet] xylene substitute

I am taking a chance that this is still a working email!! I came across an old post on histonet regarding Xylene subs with a linear stainer. I was wondering if you made the switch, and if so could you share the brand and your H&E protocol. I have had some issues with Eosin bleeding, slides very eo

[Histonet] Stainer Slide Clips

Looking for slide clips that will be compatible for our stainers. Does anyone use different vendors other than the equipment manufacturer? We have a Thermo GLX Linear Stainer. They sell 25/$144.50 . Crazy but they are necessary! V.Avalos ADS, INC Fax:602-277-2134 _

[Histonet] H&E Stain

I stain derm tissue. We do H&E only using a linear stainer. Two of my docs seem to say that stain is very eosinophilic & smudgy. Other Two say not enough Eosin. Of course!! Followed is my stain protocol. This week I have eliminated the Define and slides seem to not look as eosinophilic and I hav

RE: [Histonet] Does anyone have and use Shandon Excelsior EX

I have been using the Excelsior for over 4 yrs . Compared to the last VIP we had this is wonderful!! We save on Reagents by rotating on the first Alcohol quality. Very user friendly, Would suggest purchasing the Warranty & Netmon. A bit expensive but the PM & any parts alone pretty much cover that

FW: [Histonet] Coverslipping with SubX

all the helpful suggestions everyone! -Original Message- From: Breeden, Sara [mailto:sbree...@nmda.nmsu.edu] Sent: Tuesday, March 30, 2010 11:25 AM To: Vanessa Avalos Subject: RE: [Histonet] Coverslipping with SubX Try thinning out the Sub-X-based mounting medium with some Sub-X. It

[Histonet] Coverslipping with SubX

Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a

[Histonet] slide template

Would anyone have a template for a ½ sheet of 15/16 x 15/16 microscope slide labels? Creating templates is not something I am very good at. Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe

[Histonet] UNSUBSCRIBE

I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS! :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ma

FW: [Histonet] How to unsubscribe

Oh yes, I have gone to the link, gone to the home page and followed all of the instructions for unsubscribing. Replied with the code it gives you, have been given a confirmation email and still I am flooded with this. Have done that twice and posted it three times now. I added my work email to thi

[Histonet] SubX

I am really wanting to end the use of Xylene in our lab and considering the use of SubX from Surgipath/leica. I am currently using Gill III from Surgipath as well. I was wondering if anyone out there is also using SubX and if you would share your staining and processing regimen with me and any f

[Histonet] SLIDE LABELS

Where does everyone buy slide labels from? Am interested in printing out our own instead of getting them pre printed. Looking for 8 ½ x11 sheet of 15/16 x 15/16 labels. White and colored. Thanks in advance for any suggestions. Vanessa Avalos Histology Fax: 602-277-2134