Re: [Histonet] Retic staining

Hi Charles, Ammoniacal Silver is a tricky step, we've had a lot of repeat Bielschowsky stains in the past due to it. You don't need to go drop by drop, what's key is to gently stir the solution as you slowly add ammonium hydroxide until the precipitate is reincorporated back into the solution.

Re: [Histonet] Air pocket in paraffin blocks

Hello Vikki, What you are experiencing is the lack of quality feedback. The "experienced" techs may have never been told that their quality needs to improve due to complacency or management is afraid they might leave. This gets worse if they have gotten away with producing poor quality for a lo

histonet@lists.utsouthwestern.edu

Hello Histonetters. I'm looking for opinions from users of Cerner's Advanced Barcode labeling and Tracking Software. I'd like to know your experience working with the software, and if you've worked with another software. Thank you. Jose de Guzman __

Re: [Histonet] AFB Stain on Cytospin Preps

Greg, I'm not sure if anyone has responded to your question, the waxy coat of acid-fast bacilli is sensitive to xylene and alcohol not to acid. Carbol Fuchsin is soluble in acid alcohol but if the waxy coat of the bacteria is intact, it will hold the stain. What I've suggested before is a cyto-

[Histonet] DAKO FLEX slides

Hello Histonetters. Has anybody else encountered problems with the DAKO FLEX slides where paraffin sections are not adhering to the slide? J -- MedStar Health is a not-for-profit, integrated healthcare delivery system, the lar

Re: [Histonet] Sea urchin Spines

Have you tried urine or something similar? I know that urine dissolves the spine. Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Ri

Re: [Histonet] Nuclear Bubbling

Jim, Are you drying the slides in an oven before the heat stage on the Prisma? If you use an oven, what is the temperature setting and for how long are slides kept in? Carson and Hladik recommends 60 degrees for less than 1 hour. You mentioned, recycled formalin, what is the pH of the formalin?

Re: [Histonet] Tissue Flotation bath

Cardinal Health Cat#: M7654-1A MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachmen

Re: [Histonet] are you desperate to hire a BS.c graduate

Please correct me if I'm wrong but this has been my experience so far. I came from a research Histology background where I learned many skills that I was able to developed in the clinical side. I spent 3 years preparing for the exam because my manager in research and a co-workers failed on their

Re: [Histonet] Dako Artisan Special Stainer

Hi all, We recently upgraded our old Artisan to the Artisan Link Pro. It does the depar. within the system itself. Set-up was easy, we just transferred our old protocols to the new units. Modifying protocols was easy too. Staining is consistent, tech support and their specialists are reliable a