Dear sirs. I don't know whether I send this question to you.
For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um in crystat and dried for 1~2H at RT. 4. store at -20 5. let it dry for 30mins at RT 6. immerse it in cold 10% NBF for 10mins 7. staining After staining, my section were fallen off and seperated. I search many protocols. Some suggest additional fixation is necessary as soon as cutting. Others suggest prefixed tissue is not necessary additional fixation. Plz send me your comments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
