Re: [Histonet] Hematoxylin Precipitate

2015-09-21 Thread nancy lowen via Histonet
Yes, I have had the same experience.Filtering  it seems to help. On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet wrote: Hello all,                 Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for

Re: [Histonet] RE: Masson Trichrome stain

2015-03-13 Thread nancy lowen
Just out of curiosity--what strength of Picric acid is in your Bouins fixative?Nancy On Thursday, March 12, 2015 2:02 PM, Gayle Callis wrote: I have been following the string of inquiries about using metal forceps with Masson's Trichrome staining.  I was taught many years ago to av

Re: [Histonet] Water/Bubbles

2014-08-08 Thread nancy lowen
I have also had this problem recently. Nancy Lowen On Thursday, August 7, 2014 1:23 PM, Julie Bowman wrote: Hello all, Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplet

Re: [Histonet] RE: Embedding Paraffin

2013-11-14 Thread nancy lowen
I use McCormick Paraplast Plus. On Tuesday, November 12, 2013 8:14 AM, "Goins, Tresa" wrote: Paraplast X-tra by McCormick. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Tues

Re: [Histonet] RE: Submitting Questions

2013-09-25 Thread nancy lowen
Me too! On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same pro

Re: [Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation?

2012-11-26 Thread nancy lowen
are filled with woe and wringing our collective histology hands.                 Any recommendations for a quality rabbit/mouse polymer detection kit would be most appreciated. Thank you, Sandy Biocare Medical has one. Nancy Lowen ___ H

Re: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model

2012-10-19 Thread nancy lowen
outhwestern.edu/mailman/listinfo/histonet I use The antibody from Biocare Medical, and I also purchase the Kit that is designed for this antibody. Works well, even on bone. Nancy Lowen ___ Histonet mailing list Histonet@lists.utsouthw

[Histonet] WNT-16 Antibody

2012-04-24 Thread nancy lowen
Hoping someone can help- Does anyone know of a WNT-16 antibody that will work on mouse tissue for IHC? Thanks, nancy.lo...@va.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] A big thanks

2012-04-16 Thread nancy lowen
I would like to say a big thank you to everyone who replied and sent me ideas to get the wrinkles out of the diaphysis of the mouse tibias I was cutting. Will be trying out some of the tips I received. Thanks again Nancy Lowen ___ Histonet mailing list

[Histonet] Wrinkles in whole bone mouse tibias

2012-04-13 Thread nancy lowen
Hello to all, I am having on ongoing problem with wrinkles in the midshaft marrow area of sections of whole mouse tibias.  Leaving them in the waterbath longer does not help--water bath temp. is 48 to 49 degrees.  I have tried several waxes to help, and am currently using McCormick Paraplast Plu

Re: Fwd: [Histonet] Humidity levels and IHC staining

2012-02-17 Thread nancy lowen
We have the Lab Vision 360 from Thermo Fisher and we have really bad problems with the slides drying out.  We put wet paper towels and water boats in, but after 20 or 30 minutes, the slides have very little liquid on them.  I have gone back a lot to manual staining except for stains that work at

Re: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape?

2012-01-23 Thread nancy lowen
I also use the CryoJane and have problems similar to yours.  I get much better bone sections with it, but  they never seem to be perfect like others say they get.  I also have tried many different ways to do it, but there always seems to be a part that does not completely transfer from the tape

[Histonet] Paraffin

2012-01-12 Thread nancy lowen
I was just wondering what Paraffin most people think is the best for all around embedding, including bone samples. What cuts better, stretches better, etc. Thanks, nancy.lo...@med.va.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://

[Histonet] Collagen 111

2009-03-25 Thread nancy lowen
have got weak results trying Hyaluronidase, Prot. K, and Trypsin. Any help would be appreciated. Thanks, Nancy Lowen clayca...@yahoo.net Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

Re: [Histonet] Nuclear fast red

2009-02-25 Thread nancy lowen
Accurate Chemical and Scientific Corp.  50 ml. bottles.  Our VWR rep found it for us. Nancy nancy.lo...@med.va.gov --- On Wed, 2/25/09, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Nuclear fast red To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 25,

Re: [Histonet] CD31 on FFPE mouse tissue

2009-02-13 Thread nancy lowen
I have used the antibody and kit from Biocare for CD31 and got good results.  Simple to use. nancy.lo...@med.va.gov --- On Fri, 2/13/09, Jackie M O'Connor wrote: From: Jackie M O'Connor Subject: Re: [Histonet] CD31 on FFPE mouse tissue To: kmerriam2...@yahoo.com Cc: "Histonet" , histonet-boun