[Histonet] retraction of mounting medium

Has anyone experienced retraction of mounting medium from the edges of the slides (not yet reaching tissue but the retraction could conceivably continue until tissue is compromised) after long term (greater than 3 years) storage using recommended mounting media for automatic coverslippers? How hav

[Histonet] Randomized Curling of Thick (40um) Frozen Sections on Positively Charged Slides

Hello Histo-World, We are experiencing randomized curling of our thick (40um) frozen mouse brain sections. This curling occurs in the cortex and hippocampus region (these are coronal sections) during the counterstain process for Cresyl Stain (when the tissue is hydrated post alcohol gradient). Is

[Histonet] preserving hydrogel in tissue sections

We were asked to perform histology on goat knee joints (fixed in 10%NBF) to demonstrate a polymeric material - hydrogel. Could someone please share their procedure or direct me to someone who could help me? I couldn't find much information using PEG and are open to using other methods. We would

[Histonet] Hospitalities

Has anyone heard if there is going to be any hospitalities at the convention in Seattle this year? We haven't seen anything listed on the NSH home page yet. Thanks in advance, Paul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists

[Histonet] waterbath residue

Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on

[Histonet] position available in Northern Virginia

Histology technician position available in busy northern Virginia laboratory. Experience in a high quality/production lab and necropsy helpful but not required. Please reply to pathology.histol...@gmail.com Thanks ___ Histonet mailing list Histonet@list