I'm old, old school too. I prefer the paraffin pool myself, it helps prevent
the bon-bon effect. I had a vendor rep tell me the holding bins were designed
for molten paraffin.Probably what happened over the years is that if there's
too much molten wax in the bin, it over flows when the basket
Hi Carl,The bon-bon effect. This can happen if the wax used for processing is
different from the wax used for infiltration or when the tissue gets cold
before embedding.Either way, because the tissue is cold - the embedding wax
doesn't blend with the tissue wax, creating the hard outer wax shel
Happy Friday Eve,
We use the Sakura glas2 cover slipper. We run hundreds of slides through them
every day with very few problems.
Any automated coverslipper can have issues. A word of warning- watching the g2
do its thing is hypnotic.
Paula
Sent from my iPhone
> On May 13, 2021, at 6:18
Hi Heather,
I can see why you’re having trouble. 30 micron sections are inherently
unstable. Like paraffin, the thicker a section is the more difficult it is to
cut. Plus since your practice samples are old they tend to be more brittle.
Try cutting at 5 microns and see what happens. Remember
We used to use a drying oven set at 37 degrees C. We stopped using it because
some people thought it smelled, plus our slide volume increased to the point
where all the flats couldn’t fit.
Now we dry them for two days, minimum, before filing. I prefer to let them dry
for at least 4 days.
Good Morning Heather,
I have some questions about how you cut frozen brains.
What temperature are you cutting at?
How thick are your sections?
How are your samples frozen? Flash freezing, slow freezing, iso-pentane in
LN2?
Your answers may provide clues to help you get better cryosections
I’m not completely sure but I think you can do either a mini validation or a
comparison validation to show there’s no difference between the results.
I think CAP has a requirement for what to do when reagents change.
Paula
Sent from my iPhone
> On Mar 25, 2021, at 6:19 AM, Jeanine Ronkows
Hi Erin,
Often heat is applied to formalin to speed up fixation. That said there is
probably a temperature point where it goes from fixing tissue to cooking it.
Paula
Sent from my iPhone
> On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet
> wrote:
>
> Hello everyone!
>
> We have
Hi Garrey,
The answer is “it depends”. What you do when a processor fails depends on the
failure point. If the tissue is still in dehydrant it gets treated differently
than if it fails in the intermediate solvent.
Paula
Sent from my iPhone
> On Jul 4, 2020, at 10:08 AM, Garrey Faller via
I favor the VIP too. My lab has 4 VIPs and 1 Leica Peloris. The VIPs can
process more cassettes on a run and cost a lot less than the Peloris.
Paula
Sent from my iPhone
> On Mar 6, 2020, at 12:34 AM, warda hassan via Histonet
> wrote:
>
> Hello to all histonets
>
> Advanced thanking t
Hi Carole,
Sorry but you need to validate the change completely. Reagent alcohols are
blends that sometimes don’t even contain ethanol. You need to demonstrate
that it doesn’t affect routine H&Es, special stains, and IHC. Plus you have
to test the major tissue types you routinely work on.
This is an issue that continues to pop up. It all starts with the CLIA
regulations that do not recognize histology professionals as equal to other
laboratory professionals. Until the feds change CLIA we will keep having this
problem.
I do believe that NSH is petitioning on our behalf to c
It could be the concentration of the antibody is too high. Have you tried a
lower dilution?
What species made the antibody? I know that sounds a bit basic but I know that
I have used a mouse or rat antibody by mistake.
Paula
Sent from my iPhone
> On Nov 15, 2018, at 4:44 AM, Kooijman, E.J
Also, the manufacturers make the threads different so what works on one brand
won’t work on another. They even change the threading between models so you
have to buy new objectives.
Paula
Sent from my iPhone
> On Jun 19, 2018, at 6:44 AM, Rene J Buesa via Histonet
> wrote:
>
> Not all ob
My comment was meant in jest. I live in San Diego, so Modesto is not a city I
would vacation. Modesto probably has many nice features.
Paula
Sent from my iPhone
> On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet
> wrote:
>
> Hi Histonetters!
> I just want to take a minute to address
And the winner for the water of choice for H&E strainers is..
What ever you have available. Tap, tap with a filter, deionized.
It all depends on the water quality in your area.
Thanks to all who replied.
Paula
Sent from my iPhone
> On Sep 27, 2016, at 9:54 AM, Elizabeth Chlipala wr
I heard everything on the intervention is true, is that true?
Paula ;-)
Sent from my iPhone
> On Aug 21, 2015, at 9:16 AM, Jay Lundgren via Histonet
> wrote:
>
> There's this cool thing called the Interwebz now?
>
> http://www.amazon.com/Multi-Purpose-Freeze-Spray-1-Ea/dp/B0017UIB30#Ask
>
>
Hi Tonya,
Soak the brick-o-slides in xylene until they unstick from each other.
This can take over a week, you must be patient. The slides will break if you
try to force them apart.
Paula
Sent from my iPhone
> On Aug 10, 2015, at 6:51 AM, Abbott, Tanya via Histonet
> wrote:
>
> We found
I was told by the technical specialist to make the wash solution fresh before
use. There has been a contamination caused by growth of organisms in the wash
if it sits around too long between uses.
Paula :-)
Sent from my iPhone
> On Jul 16, 2015, at 10:14 AM, Blake Taylor via Histonet
> wrot
19 matches
Mail list logo