Hi please remove me from the mailing list
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From: "Vanessa Avalos";
Date: Thu, Apr 7, 2011 05:54 AM
To: "HISTONET LISTS";
Subject: RE: [Histonet] Slides sent out for outside consult.
Our practice does not bill the patient. Before sendin
hi if u use triton for permeablization in IHC
it sometimes cause the stain pattern to diffuse from nucleus to cytoplasm
it is common
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From: "Kim Merriam";
Date: Thu, Apr 15, 2010 07:38 PM
To: "Thotakura, Anil Kumar";
"Histonet";
Very surprising!
I just use PFA fixation after I cut the tissue, and I can stain it again!
Anyone has the experience of staining IBA1 on alcohol/acetone-fixed brain
sections before?
In literature it said yes this works.
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From: "TF"
Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig
secondaryantibody on rat tissue
it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody
from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on
blood vessel-like structures.
it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody
from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on
blood vessel-like structures.
Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross
react with rat IgG ?
Or it
never store everything in one cabinet.
As far as i know, HCl and formalin should not be stored together. If any of
you know why...
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From: "Riesen, Rebecca";
Date: Fri, Sep 11, 2009 10:09 PM
To: "histonet";
Subject: [Histonet] formalin
How to label the antibody with FITC?
it is hard, or a complex procedure
-- Original --
From: "Andrea Hooper";
Date: Tue, Sep 8, 2009 03:09 AM
To: "Histonet";
Subject: [Histonet] RE: Staining with two primary antibodies from same host
I routi
