The first things to check are 1. adequate fixation. 2. Complete removal
of paraffin.
That done, I don't think you can use staining intensity to draw any
conclusions about the health, or lack thereof, of collagen.
I suggest finding some references on collagen production and degradation
before pro
I have an investigator looking at Massons(with Aniline Blue), Van Gieson
and Gomori's all showing the same type of collagen staining. In some
areas collagen fibers are brilliantly stained and other fibers are pale
all on the same slide. This is consistent across the board so we know
it is not the
