This is in response to a question about the ACE stain for Hirschprung's.  Here 
at Penn State University M.S. Hershey Medical Center we still do the ACE stain 
using a kit from MBL (Medical and Biological Laboratories CO., LTD.) The 
instructions come with the kit.  It provides enough reagents to do 30 tests. It 
is relatively easy to do and takes about 1/2 hour from start to finish. The kit 
is a bit pricey but we used to have a procedure that required us to keep all 
kinds of little used chemicals around and we felt that this was a safer option. 
Donna Rossi, HT(ASCP)
.


Donna J. Rossi                                    
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