[Histonet] Air Bubbles

2016-07-22 Thread Charles Riley via Histonet
Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I

Re: [Histonet] Air Bubbles

2016-06-10 Thread Barry Rittman via Histonet
media down the entire slide not just 2 or 3 drops. Might > help.Karen PfaffLead HT Skin Cancer CenterMohs lab > > Sent from my U.S. Cellular® Smartphone > > Original message > From: Charles Riley via Histonet > Date: 06/10/2016 9:00 AM (GMT-06:00) > To:

Re: [Histonet] Air Bubbles

2016-06-10 Thread Karen Pfaff via Histonet
. Cellular® Smartphone Original message From: Charles Riley via Histonet Date: 06/10/2016 9:00 AM (GMT-06:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air Bubbles Hello everyone, Recently we have been having an issue with a majority of our slides having air

[Histonet] Air Bubbles

2016-06-10 Thread Charles Riley via Histonet
Hello everyone, Recently we have been having an issue with a majority of our slides having air bubbles under the coverslips. When they are originally coverslipped there are no bubbles and any present are pushed out and the sides are all wiped dry. We have not changed any reagent or processes in ov

RE: [Histonet] Air bubbles in Coverslipper

2013-08-04 Thread Sanders, Jeanine (CDC/OID/NCEZID)
@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium

RE: [Histonet] Air bubbles in Coverslipper

2013-08-03 Thread Rittman, Barry R
'gu.l...@gmx.at'; 'Anna Huntley Coffey' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Air bubbles in Coverslipper We have had success with this also. If you got the unit new, I believe that there should have been one extra bottle with it. -Original Message

RE: [Histonet] Air bubbles in Coverslipper

2013-08-03 Thread Rathborne, Toni
, August 03, 2013 5:16 AM To: 'Anna Huntley Coffey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Air bubbles in Coverslipper Hi, We always have more than one medium-bottle prepared. At least three are filled in advance. So entrapped air can go away until usage and the "

AW: [Histonet] Air bubbles in Coverslipper

2013-08-03 Thread Gudrun Lang
tonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley Coffey Gesendet: Freitag, 02. August 2013 21:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) ha

[Histonet] Air bubbles in Coverslipper

2013-08-02 Thread Anna Huntley Coffey
Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to c