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- Original Message - From: "RICKY MATHIS"
To: "histonet"
Sent: Wednesday, March 17, 2010 8:15 PM
Subject: [Histonet
Y MATHIS"
To: "histonet"
Sent: Wednesday, March 17, 2010 8:15 PM
Subject: [Histonet] Anti-human abs work on porcine tissue
I work with some porcine tissues and it is sometimes difficult to find
antibodies specific to pig. A doctor I work with asked some one he knew in
Europe about
I work on porcine tissue regularly and I can't confirm the 10%.
Maybe I accidentially used only the highly conserved proteins, I didn't
check on that.
But I usually try anti-human antibodies and most of the time they work
really well!
Alexandra
Dr
I routine use anti-human antibodies to stain mouse tissue, so it can be
done. It completely depends on the antigen they used to immunize. If the
human and pig proteins are highly homologous or identical, then it has a
good chance of working. If they're not, you're in uncharted territories. If
you a
I work with some porcine tissues and it is sometimes difficult to find
antibodies specific to pig. A doctor I work with asked some one he knew in
Europe about using antibodies that are listed as working in human being used on
porcine tissue. This person stated that there would be about a 10% c
