Here a protocol for Bouin fixed tissue and it is working on testis.
TUNEL analysis on Bouin fixed, paraffin embedded tissue
* Incubation box in stove (37°C) with wet tissues.
* Dewax. All steps 3 min 3x Xylene, 2x Ehanol 100%, 1x Ethanol 96%, 1x
Ethanol 70% and 1x MilliQ water.
* Mi
Hello all,
I am trying to stain for apoptosis on bouins fixed, paraffin embedded mouse
testis. I have tried a cleaved caspase-3 anitbody, & TUNEL, with no success.
Any suggestions would be much appreciated.
Many thanks
Lindsey
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