Ion Exchange Decal containers will change your experience forever.
-Original Message-
From: Gudrun Lang via Histonet
Sent: Friday, January 26, 2024 3:32 AM
To: 'Chakib Boussahmain'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone samples
Hi,
In my o
en to get rid of any
residual water under the section.
Hope this helps
Gudrun
-Ursprüngliche Nachricht-
Von: Chakib Boussahmain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Mittwoch, 24. Jänner 2024 23:34
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone sa
Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu
-Original Message-
From: Chakib Boussahmain via Histonet
Sent: Wednesday, January 24, 2024 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone samples (EXTERNAL EMAIL)
CAUTION: BE CAREF
Hi guys,
I hope this messagefinds you well. I am currently working on a study involving
bone samples thathave been treated with slow decal and embedded in paraffin. I
am facingchallenges in obtaining nice sections, and I was wondering if you
could providesome guidance or recommendations.
Tha
We have used the IMEB band saw for years and love the results. It has a
similar footprint and will cut through anything, including a steel pin. It
also has a diamond blade and optional water cooling. It does seem a little
awkward to clean, and I wish the protective housing was a little better
Martha,
We reported our study of 15 brands of adhesive slides for "wash off" and
found little difference among the different slides when well-fixed cell
culture material was examined.
On the other hand, poorly-fixed breast cancer tissues did appear to adhere
more strongly to some slides than other
It was brought to my attention that we had significant washing on 3 of 8 bone
marrow clot sections the other day; this is not the first time so we would like
to get to the bottom of this. We use positively charged slides and all 8
cases were cut and run the same morning but allowed to air dry
In my experience soaking the block in water works just as well as surface
decal. If you need to you can take the bone back, by reverse processing. Run
the block through the clean cycle back to water, then leave the bone in NBF for
24-48hrs. The fixative will protect the tissue when you put it ba
Use surface decalcification:
Surface the block, then remove from the microtome. Do not use the
microtome for another block, so as to avoid adjusting the position of
the block face to the knife.
Place the block face down in 4% nitric acid for 2 hours or longer.
Rinse the block and cool down
To Histopeeps,
Does anyone know if there is a method to decalcify bone once it is FFPE?
Thank you,
Pat L
George Washington University
Washington, DC
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Our Histology Techs also assist with Bone Marrows in CT, though there is little
to do. We collect the aspirate in Heparin Tubes and the core in Bouins. We
bring it back to the lab and make our smears from one of the heparin tubes.
The other tubes go for special studies. I'd like to think our
Can someone please recommend a good place to order customizable bone marrow
biopsy kits?
We want foam for specimen containers, tubes, and want to add our own
products. Not premade completely.
Thank you,
JB
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From: MANTERO Stefano RIC
Sent: Thursday, February 21, 2019 7:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone PMMA sections
Good morning Histonetters!
I'm starting to cut out of bone samples included in PMMA.
I would like some suggestion how to storage the slides cut.
Good morning Histonetters!
I'm starting to cut out of bone samples included in PMMA.
I would like some suggestion how to storage the slides cut.
Many thanks in advance
Stefano
Dott. Stefano Mantero
Human Genome Laboratory CNR-IRGB
c/o Humanitas Research Hospital
Via Rita Levi Montalcini (Ex
Hello. Does anyone have a processing protocol for formalin fixed decalcified
rat bones using an automated processor? I've been doing it manually with a
vacuum oven and it takes 14-16 hours to complete. I don't have a vacuumed
section on my automatic processor.
Also any tips besides what I've re
>
> Terri L. Braud, HT(ASCP). Anatomic Pathology Supervisor, Holy Redeemer
> Hospital, Meadowbrook PA describes:
>
> >>We use an awesome little band saw made by IMEB, Inc. It has a small
> foot print, 4 blade types and added accessories for a super lab bone
> cutting station, and best of all, very
I agree, we use the IMEB bone saw as well. All human bones though...
-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, October 27, 2016 10:34 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Bone saw
en Sweeney)
--
Message: 6
Date: Thu, 27 Oct 2016 16:55:06 +
From: Lauren Sweeney
Subject: [Histonet] bone saw
Hello histoworld,
Does anyone out there use a bone saw in their lab? We routinely have research
cases with hundreds of femur head submis
Hello histoworld,
Does anyone out there use a bone saw in their lab? We routinely have research
cases with hundreds of femur head submissions from avian species. We currently
use a bone saw made by Buehler from the 70's or 80's and it's a work horse, but
the blade keeps cracking in the diamond
Hi all, in our lab we have a Buehler Isomet Low Speed Saw that looks like it is
from the 80's. Up until about 7 months ago, we have used the bone saw fairly
infrequently. As far as I know, this bone saw wafering blade has never been
replaced. We now have a researcher who regularly needs sections
: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] bone marrow clots
Hi all,
Question: Does anyone produce beautiful, well fixed bone marrow clot blocks
that has a procedure they would be willing to share? Do you all use the
specimen from the EDTA tube after prepping the smears
Hi all,
Question: Does anyone produce beautiful, well fixed bone marrow clot blocks
that has a procedure they would be willing to share? Do you all use the
specimen from the EDTA tube after prepping the smears? Any help would be
greatly appreciated!
Thank you,
Noëlle
Noëlle Linke, MS, HTL(A
Hi All,
I am not having much luck finding exact information to perform bone density on
rat femurs/tibias using Archimedes principle. Can anyone provide an exact
procedure, or point me in the right direction? Including exact type of
equipment used, etc. I have found many references with outlin
Hi All,
Are you allowed to bill an 88305 for both the aspirate and the biopsy on bone
marrow cases?
Thank you,
Lacie
Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...
Hi All,
Can any one let me know general price for mouse bone decalcification,
processing, microtome charges for per block or per slide.
it will great help.
Many thanks,
NOTICE
Please consider the environment before printing this email. This message and
any attachments are intended for th
Hi Everyone,
I was wondering if anyone is billing for a Histo technical charge on bone
marrow aspirates and biopsies (i.e. an 88305) in addition to all of the
Hematology fee codes. Our Histology department does the grossing, embedding
and microtomy for all bone marrow blocks, however we do not
How are you billing for bone marrow bxs. we do irons on smear and core plus
clot. should we bill for all iron stains? also we do a wrights stain.
Anita Dudley
Providence Hospital
Mobile, Al
___
Histonet ma
Original Message-
> From: Rathborne, Toni [mailto:toni.rathbo...@rwjuh.edu]
> Sent: Tuesday, February 17, 2015 2:55 PM
> To: 'Jason McGough'; Mike Pence; histonet-boun...@lists.utsouthwestern.edu;
> histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Bone S
From: Rathborne, Toni [mailto:toni.rathbo...@rwjuh.edu]
Sent: Tuesday, February 17, 2015 2:55 PM
To: 'Jason McGough'; Mike Pence; histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Bone Saw
We use a device from Mopec.
htt
esday, February 17, 2015 3:59 PM
To: Mike Pence; histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Bone Saw
We use a Dremel tool. It works great!!
Jason McGough, HT(ASCP)
Operations Manager
Clinical Laboratory of the Black Hills
605-343-2267
ence mailto:mpe...@grhs.net> >
> Sent: Tuesday, February 17, 2015 1:56 PM
> To: histonet-boun...@lists.utsouthwestern.edu
> <mailto:histonet-boun...@lists.utsouthwestern.edu> ;
> histonet@lists.utsouthwestern.edu <mailto:histonet@lists.utsouthwestern.edu>
> Subj
I am trying to see what everyone is using at your grossing station for bone saw
to cut femoral heads and toes for osteo. If you are using a Stryker saw how are
you holding the specimens to make good thin sections?
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Dear Histonetter's,
Happy new year to everyone! I come today with a couple of questions regarding
human bone embedded in MMA:
1. What is a good technique to dissolve the MMA from the tissue section?
Also any suggestions on how to stain with H&E?
2. After mounting my tissue sect
Hi Joana,
We are currently using the Milestone microwave to decal our bone marrow core
biopsies (for faster TAT). We use our in-house formic acid (10%) and run it in
the microwave for 2.5 to 3.00 hours with 50 degree Celsius cycle (you can tweak
to where it works for your specimens). Assuming
...@ihctech.net
> Date: Mon, 17 Nov 2014 14:05:28 -0500
> From: carolyn.barn...@va.gov
> To: Histonet@lists.utsouthwestern.edu
> CC:
> Subject: [Histonet] Bone Marrow processing using Immunocal
>
> I am looking for a Bone Marrow procedure that uses Immunocal as the
> decalc
I am looking for a Bone Marrow procedure that uses Immunocal as the
decalcifier. I am particularly interested in how long they fix in
Immunocal and if heat is used in any way . Our lab just started using
this product because we are under the impression that it gave better ISH
results.
Thank-you a
Nov 2014 09:28:01 -0600
From: Nicole Cosenza
Subject: [Histonet] bone paraffin embedding
To: "histonet@lists.utsouthwestern.edu"
Message-ID: <54579f01.3070...@siumed.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Histonetters:
Our lab needs to paraff
, tips and tricks,
etc.
Best,
Jack
From: ratliffj...@hotmail.com
To: ncose...@siumed.edu; histonet@lists.utsouthwestern.edu
CC: sarah_m...@urmc.rochester.edu; k...@nsh.org; ratliffj...@gmail.com
Subject: RE: [Histonet] bone paraffin embedding
Date: Mon, 3 Nov 2014 12:07:06 -0500
Nicole,
You can
n, 3 Nov 2014 09:28:01 -0600
> From: ncose...@siumed.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] bone paraffin embedding
>
> Histonetters:
>
> Our lab needs to paraffin embed and cut bone. Is there a special
> process for fixation of bone, or can i
: Monday, November 03, 2014 9:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone paraffin embedding
Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special process for
fixation of bone, or can it be harvested and dropped right into NBF?
--
Nicole Cosenza
Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special
process for fixation of bone, or can it be harvested and dropped right
into NBF?
--
Nicole Cosenza
Research Technician
Institute for Plastic Surgery
SIU School of Medicine
Springfield, Il
217.545.3862
__
Hi Histonetters,
I am looking for a bone saw for smaller surgical specimens (like jaw bone). Any
suggestions or input about what is working or not working for you would be
helpful.
Thanks in advance!
This email and/or any documents in this transmission is intended for the
addressee(s) only a
Hi Merissa,
Exakt technologies makes a wonderful saw designed specifically for exactly what
you are trying to do with a hack saw. It is a bit pricy though. Contact Linda
Durbin at 405-848-5800 for a quote.
Alternatively, you can use a wet saw designed for cutting stained glass. Check
out th
-6508
From: histonet-boun...@lists.utsouthwestern.edu
on behalf of M.O.
Sent: Tuesday, August 19, 2014 3:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone saw for cutting slabs
Histoland! Happy Tuesday!
I just wanted to get
Histoland! Happy Tuesday!
I just wanted to get your feedback on cutting slabs from human femora
for histopathological analysis.
At them moment we are just using a hack saw to cut 7mm slabs from
femora. We notice some marks on the cartilage from sawing, so when we cut
the tissue down af
Over the past several years we have had some questions by our
hematopathologists regarding the quality of the trephine blocks. The biggest
concern has been that the sections seem to have a fragmented appearance which
we have determined in the past to be areas where the lipid cell borders brea
Has anyone experienced poor staining of bone marrow smears that were sent from
a doctor's office to a central laboratory? We have some cases of poor staining
that we are thinking may be from fume contamination from the fixative bottle.
Has anyone experienced anything of this nature? Thank you fo
Hi All , I was wondering if anyone had any advise on getting clots and cores to
stay put? We are using plus slides and giving the slides extra oven time and we
are still having issues. So if anyone has some histomagic up their sleeve
regarding this issue it would be appreciated.
Ginny Miller HT
Trevor, Feel free to contact me. I have worked with decalcified bone
(undecalcified, as well) for a number of year. Vicki
Vicki Kalscheur
Department of Surgical Sciences
School of Veterinary Medicine
University of Wisconsin
2015 Linden Drive
Madison, WI 53706-1102
Phone: 608-262-8534
FAX: 608-263-
an
Cc: histonet@lists.utsouthwestern.edu; Jack Ratliff
Subject: Re: [Histonet] Bone Histology Protocol
Trevor,
May I ask first if there is a particular reason why you are wanting to
decalcify? Have you ever considered resin/plastic embedding of non-decalcified
bone? Also, what type/species of bon
an Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S. Biochemistry
From: Jack Ratliff
Sent: Saturday, March 1, 2014 3:16 AM
To: Wait, Trevor Jordan
Cc: histonet@lists.utsouthwestern.edu; Jack Ratliff
Subject: Re:
Trevor,
May I ask first if there is a particular reason why you are wanting to
decalcify? Have you ever considered resin/plastic embedding of non-decalcified
bone? Also, what type/species of bone are you wanting to process?
My name is Jack Ratliff and I Chair the Hard Tissue Committee for the N
Hello colleagues! I'm currently trying to construct a complete Bone Processing
Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration,
Clearing of the decalcificant (Xylene), infiltration, and Embedding in
Paraffin. I would like to look at some procedures just to get a goo
Research Biologist
>> 636-926-7476 phone
>> terra.wine...@novusint.com
>>
>>
>> -Original Message-
>> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
>> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net
>> Sent: Thu
: histonet@lists.utsouthwestern.edu; Wineman, Terra
Subject: Re: AW: [Histonet] Bone samples long-term storage in 10% formalin
or 4% paraformaldehyde
hi
I would recommend storage for long term in 70% ethanol. To prevent drying
out we used glycerin in the ethanol, about 20% of the volume.
Barry
O
t; > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> pru...@ihctech.net
> > Sent: Thursday, December 05, 2013 2:50 PM
> > To: gu.l...@gmx.at; 'Orla M Gallagher&#
:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net
> Sent: Thursday, December 05, 2013 2:50 PM
> To: gu.l...@gmx.at; 'Orla M Gallagher'
> Cc: histonet@lists.utsouthwestern.edu
> Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% for
To: gu.l...@gmx.at; 'Orla M Gallagher'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or
4% paraformaldehyde
i would think u are correct in advising formic acid decal and then processing
into paraffin for the best protect
activity for TRAP
that may have been lost so if u need that let me know.
- Original Message - Subject: AW: [Histonet] Bone samples
long-term storage in 10% formalin or 4% paraformaldehyde
From: "Gudrun Lang"
Date: 12/5/13 11:42 am
To: "'Orla M Galla
Orla M
Gallagher
Gesendet: Donnerstag, 05. Dezember 2013 19:31
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4%
paraformaldehyde
Dear Histonetters,
What is your opinion on storing bone samples long-term (more than a couple
of weeks) in
Dear Histonetters,
What is your opinion on storing bone samples long-term (more than a couple
of weeks) in 10% formalin? As I was taught, best practice has always been
to fix only as long as necessary, depending on the size of the sample, then
decalcify and process to wax, and I always stress this
A H&E will allow you to identify those three tissue types, in normal tissues,
of most organisms ( certainly
in avian embryos, imho), IF you know your Histology.
There is no stain/demonstration technique that will substitute for this
knowledge, imho.
Sure, a "special stain" will help to confir
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rui TAHARA
Gesendet: Freitag, 23. August 2013 09:49
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone/cartilage/epithelial tissue stain
Hi,
Would anyone suggest me what staining is best to color differentiate
At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green. T-blue (buffered appropriately)
stains the
proteoglycan a lovely metachromatic blue and the bone and most
everything else
is green. The nuclei of cells are also green since we used no nuclear
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rui TAHARA
Sent: Friday, August 23, 2013 1:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone/cartilage/epithelial tissue stain
Hi,
Would anyone suggest me
Hi,
Would anyone suggest me what staining is
best to color differentiate between cartilage and bone and epithelial tissues
in avian embryos?
I have been trying Mallory Trichrome for
embryos but recently I was suggested that Mallory Trichrome stains cartilage
differently
in embryos compared to
NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher
Sent: Saturday, 13 July 2013 12:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone decalcifying/proce
...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher
[o.m.gallag...@sheffield.ac.uk]
Sent: Friday, July 12, 2013 8:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone decalcifying/processing problem
Dear Histonetters,
Has anyone ever seen large holes/vacuoles in the bone marrow of mouse
long bones
Dear Histonetters,
Has anyone ever seen large holes/vacuoles in the bone marrow of mouse
long bones after decalcification and processing to wax? We've had this
problem with a study which was fixed in 4% paraformaldehyde and
decalcified at 4 degrees C over a 14 day period according to a
routinely-u
I sent out a request for information a while back and am going to ask again
from a different approach.
I am finding problems with embedding blocks of bone marrow particles with or
without blood clot.
Does anyone have a special technique they would like to share to concentrate
the particles centr
the
quality of the slides.
Cathy
Kelowna, B.C.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's
Sent: Saturday, June 1, 2013 4:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone m
I am also interested in how others are processing bone marrow aspirates.
Currently, we let the aspirate clot on a watch glass for about 20 minutes,
gentle slide it onto filter paper, roll it around to get rid of the excess
fluid, place the clot between sponges, and process as usual.
However, I a
Can anyone share with me their process for processing bone marrow aspirations
and embedding them in paraffin blocks. Currently ours are being spread out
throughout the entire base mold and makes it cumbersome to screen. Has anyone
used histogel or making a button and embed that instead of just
riginal Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jordan
>Phillips
>Sent: Tuesday, May 28, 2013 7:56 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Bone Marrow Issues
>
>HI everyone.
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jordan Phillips
Sent: Tuesday, May 28, 2013 7:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone Marrow Issues
HI everyone. We are currently having problems with all of our bone marrows,
mainly clots, washing off the slides. We
HI everyone. We are currently having problems with all of our bone marrows,
mainly clots, washing off the slides. We fix our bone marrows in Zenkers. We
use positive charged slides, put the slides in the oven for a minimum of 2
hours and hand depar with no agitation. When we get to the water
Try hydrating the slide in 95% then distilled before staining. The iron stain
is made in water so hydrating to water should help the stain transfer better.
Hope this helps :)
Cassandra Davis
cda...@che-east.org
302-575-8095
Saint Francis Hospital
Saintfrancishealthcare.org
Saint Francis Facebook
Hello, We are currently having problems with our bone marrow aspirate slides
for iron stains which we run on the Ventana nexes special stainer. The RBCs
look crenated or damaged. We currently fix them 5 minutes in methanol then air
dry before running them on the stainer. Any suggestions?
Jason R
If you are currently working with bone, biomaterials or medical device
implants, you won't want to miss this specialty histology event
http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by
Polysciences Inc. and supported with six (6) continuing education units (CEU's)
Ratliff wrote:
>
>
>
> Begin forwarded message:
>
> *From:* "De La Vega Amador, Rodolfo Enrique" >
> *Date:* March 15, 2013, 9:41:23 PM GMT+01:00
> *To:* "histonet@lists.utsouthwestern.edu" <
> histonet@lists.utsouthwestern.edu>
>
Hi everybody,
I am fairly new to all Histotechnology processes. We mainly work in our lab
with mineralized bone with implants, so we fix them, dehydrate them, embed them
in MMA, cut them, glued them to slides, ground them manually and then stain
them. I need help in some parts of the process th
I fix in NBF at pH7 exactly and decalcify with EDTA
René J.
From: Clare Thornton
To: "histonet@lists.utsouthwestern.edu"
Sent: Thursday, February 14, 2013 9:22 AM
Subject: [Histonet] bone marrow specimens
What fixative and decal solution is everyone using for bone marrow specimens
What fixative and decal solution is everyone using for bone marrow specimens
which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently
using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists
demand a quick turn around time, and are willing to sacrifi
slide, the whole slide is
marrow.
Good luck!
Ashley
Message: 5
Date: Fri, 4 May 2012 16:54:57 +
From: "Coskran, Timothy M"
mailto:timothy.m.cosk...@pfizer.com>>
Subject: [Histonet] bone marrow aspirate
To:
"histonet@lists.utsouthwestern.edu<mailto:histonet@
Does anyone have a protocol on how to fix and process a bone marrow aspirate to
paraffin?
Thanks,
Tim Coskran
Pfizer
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Why not sent in 10 % formalin. Freezing it may change the morphology of the
bone to fixation after thawing. I would give that a try. After all, you are
running 3 different stains at one time in order.
Lawrence S Allen
Lead Histotechnologist
Dorm VA Medical Center
Columbia, SC
What is the procedure for collecting and processing bone marrow bx? How much
time should it be placed in DECAL??
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We assist with bone marrows either at bedside or in Outpatient Observation,
never in the OR. All other bone marrows are performed at the Oncologist's
office.
Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center
1415 Ross Ave
El Centro, CA 92243
760-339-7267
7
Yes we do. We assist with all bone marrows regardles of where they are
(not hematology). We dress out in scrubs, etc.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l
Are there any Histo Techs out there who assist with bone marrows IN the
operating room?
Lisa
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What's up Tena!
Long time. The bone marrows will be fixed in B-5.
Dawud
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Hello Histonet!
Does anyone have an H&E automated stainer protocol for bone marrows that they
wouldn't mind sharing? I'm looking at different protocols. It would be greatly
appreciated. Thanks.
Dawud
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goria
Subject: [Histonet] bone marrow biopsy
To: "Histonet@lists.utsouthwestern.edu"
Message-ID:
<
12b71261212be94bb9fb7735484b9fa1014...@exmbx01.ecrmc.ci.el-centro.ca.us>
Content-Type: text/plain; charset="us-ascii"
Hello Histoland,
I read on Histonet tha
Hello Histoland,
I read on Histonet that some of you guys use EDTA for bone marrow core bx's. I
was wondering what is the protocol? It will help us a lot. Thanks in advance!
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What hematoxylin is best for doing H&E on ground sections of undecalcified
bone- one that won't leave a precipiate on the bone?
Cindy Baranowski
SJTRI
Atlanta, GA
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
Martinez-Longoria
Sent: Thursday, March 24, 2011 11:01 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow biopsy decals
Our pathologist wants
Hi Diana,
I recently made a histonet posting "Great B5 substitute" which also
addresses BM decal. Since there are a number of companies who sell
decal solutions with similar names, I am not sure you tried the
products from BBC Chemical. It is best to use both products
together. My post
Our pathologist wants to know why Formical-4 is the best decal for bone marrow
biopsies, since we heard about in Histonet? I have experimented with four
different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid
and we are still not sure which will be the best decal. Also we a
Cal-Ex II (available from Fisher and possibly elsewhere) fixes and decals
together. Depending on the downstream tissue applications, it can be good, and
is MUCH faster than EDTA.
Ditto on the thoughts and hopes for Japan's recovery.
Jan Berry
University of Michigan
Hi Mary
We use 10% Formic Acid for our bone marrow bx and larger surgical bones (Ex:
femur, pel-vis, mandibular bones)
Thomas Huynh
From: "histonet-requ...@lists.utsouthwestern.edu"
To: histonet@lists.utsouthwestern.edu
Sent: Sat, March 12, 2011 12:01:57 PM
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