Re: [Histonet] Bouin's without the picric acid?

2011-09-14 Thread Rene J Buesa
J. --- On Wed, 9/14/11, Tyrone Genade wrote: From: Tyrone Genade Subject: [Histonet] Bouin's without the picric acid? To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 14, 2011, 7:52 AM Hello, I have been using Bouin's fixative on my fish specimens. I had simply be

Re: [Histonet] Bouin's without the picric acid?

2011-09-14 Thread Geoff McAuliffe
Hi Tyrone: Picric acid may be bad for some silver stains but not for all. Williams fix is recommended for the Bodain method and I have used it with excellent results on mouse brain. Also overnight immersion fixation of a whole fish - you did not say how large- is not going to give good results

RE: [Histonet] Bouin's without the picric acid?

2011-09-14 Thread Laurie Colbert
-boun...@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Wednesday, September 14, 2011 4:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bouin's without the picric acid? Hello, I have been using Bouin's fixative on my fish specimens. I had simply been killing the fish, slitting them d

[Histonet] Bouin's without the picric acid?

2011-09-14 Thread Tyrone Genade
Hello, I have been using Bouin's fixative on my fish specimens. I had simply been killing the fish, slitting them down their belly and letting them lie in Bouin's overnight. This worked well to soften the tissue and in particular the brain case (I'm interested in the brain but the dissection is de