Re: [Histonet] BrdU incorporating and staining

2008-09-16 Thread tf
mice for injection into the rat brain? 2008-09-16 tf 发件人: Nejat Yilmaz 发送时间: 2008-09-16 14:24:11 收件人: histonet@lists.utsouthwestern.edu 抄送: 主题: [Histonet] BrdU incorporating and staining Dear Netters, We are setting up a cell therapy model in neonate rats by injecting primary

[Histonet] BrdU incorporating and staining

2008-09-15 Thread Nejat Yilmaz
Dear Netters, We are setting up a cell therapy model in neonate rats by injecting primary cultured astrocytes intracerebrally. My questions are: 1. Is it possible to incorporate BrdU in all cells by adding it 10 uM concentration into culture media from beginning of the culture? 2. If so, can