As an additional note: paraffin oil = mineral oil.
René J.
--- On Tue, 3/9/10, Michael Folsom wrote:
From: Michael Folsom
Subject: Re: [Histonet] Butyl alcohol
To: "Krueger, Todd"
Cc: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 9, 2010, 11:17 AM
Hi:
I'm not sur
Hi:
I'm not sure if this helps but Botanists have long used tert-butanol
(not N-butanol) to embed tissue in paraffin. This classic protocol was
popularized by D. A. Johansen in the mid 1930's and involves transition
from water to alcohol to t-butanol (I can supply more detailed info if
you need
Use 2-propanol instead. It is better and much cheaper.
René J.U
--- On Mon, 3/8/10, Krueger, Todd wrote:
From: Krueger, Todd
Subject: [Histonet] Butyl alcohol
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 8, 2010, 10:00 AM
Does anyone have a procedure for processing with N-butyl
Does anyone have a procedure for processing with N-butyl Alcohol. We
need to find a procedure w/o xylene.
Thanks
Todd Krueger
HTL(ASCP)CM
Boston Scientific
2 Scimed Place, P121
Osseo, MN 55311
Phone: 763-694-5709
Fax: 763-694-5505
e-mail: todd.krue...@bsci.com
__
any
application, is its vapour. It doesn't smell nasty but it makes you cough.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: "Krueger, Todd"
Date: Wednesday, March 3, 2010 17:08
Subject: [Histonet] Butyl alcohol
To: histonet@lists.utsouthwester
Has anyone tried using butyl alcohol as a dehydrating and clearing agent
on there tissues? What are some pros and cons people have experienced
with using Butyl alcohol?
Todd Krueger
HTL(ASCP)CM
Boston Scientific
2 Scimed Place, P121
Osseo, MN 55311
Phone: 763-694-5709
Fax: 763-694-5505
e-m
