Hello, Kristyn, I don't know what species you work with but I work in mouse (mouse lupus kidneys) and had good luck with Dakos C3c antibody F0201 using Dakos Proteinase K 10 minutes at room temperature. I used a unlabeled version that is not available anymore but F0201 (FITC) one is said to be of the same clone. I had aliquot lots of the old antibody that just ran out so I have not tried this FITC one yet. If you want to stain using DAB I've used a Rabbit anti-FITC (Invitrogen) and then come back with Anti-rabbit HRP polymer then DAB. Its works nice when you have a FITC primary but don't want to visualize with Fluorescence due to background etc.. We have tested a lot of Ab's on paraffin for various epitopes and have a good success rate you just may have to test some antibodies and a few pretreatment conditions (EDTA, Citrate, PTK, Protease 1) we use a Leica Bond autostainer.
Best of luck. Jamie Erickson Scientist Abbvie bioresearch Center Worcester, Ma 01605 -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, February 08, 2016 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. C3 IF staining of FFPE tissue (Kristyn Ferber) 2. Issue with Leica/Jung Histoembedder (dayuang) 3. RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Feb 2016 08:15:21 -0500 From: Kristyn Ferber <kristyn.fer...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C3 IF staining of FFPE tissue Message-ID: <CAMnUO=bxG+f5ZyTuR2QhVt7ff3uQdhhcyTk=sJT=_lrmj8j...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hello List, I suspect it may be a lost cause (since processing likely kills the antigen site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I know frozen is ideal but occasionally, we need to revert to the FFPE sample. Best, Kristyn ------------------------------ Message: 2 Date: Mon, 8 Feb 2016 11:52:28 -0500 From: dayuang <dayu...@rci.rutgers.edu> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Issue with Leica/Jung Histoembedder Message-ID: <93d4868d-8741-4586-9f9a-0cc155d3a...@rci.rutgers.edu> Content-Type: text/plain; charset=utf-8 Dear all, We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn?t flow anymore, wax reservoir stopped heating. There was no error message displayed. Switching the power off and on corrected the problem immediately at the first time. However for the 2nd onset, we had to wait for hours until it came back. Please kindly share your experience if anybody has undergone and resolved the problem. I am more than grateful to your kind input. Best regards, Dayuan ******************************************* Dayuan Gao, Ph.D. Assistant Research Professor Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey 160 Frelinghuysen Road Piscataway, New Jersey 08854 Phone: (848)445-6837 Fax: (732)445-3134 ******************************************* ------------------------------ Message: 3 Date: Mon, 8 Feb 2016 12:44:26 -0500 From: "Pam Barker" <rel...@earthlink.net> To: <Histonet@lists.utsouthwestern.edu> Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 Message-ID: <05ce01d16298$5ac424d0$104c6e70$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histopeeps! What would make the perfect management position? ? Is it the location? ? Perhaps the type of lab? ? How about the size of the staff/lab? ? Maybe it?s the Hours? The Money and/or benefits? ? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at rel...@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Lab Manager ? Williamsburg, VA Pathology Supervisor ? Manchester, NH Histology Supervisor ? Amarillo, TX Lead Tech/Supervisor ? Fayetteville, AR Sr. Clinical Lab Specialist/Pathology ? Norfolk, VA IHC Tech Support ? Los Angeles, CA All of these clients offer autonomy in your position and a staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again!? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 8 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet