inal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 14, 2013 12:27 PM
To: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD31 problewms
If you have not changed
J.
From: "Jo-Ann Bader, Ms."
To: "histonet@lists.utsouthwestern.edu"
Sent: Thursday, February 14, 2013 11:49 AM
Subject: [Histonet] CD31 problewms
We are having problems getting our CD31 to work well on our NBF fixed mouse
tissue. There is staining but it is very pale.
We are having problems getting our CD31 to work well on our NBF fixed mouse
tissue. There is staining but it is very pale.
The CD31 comes from Bio-care and we are are using the IntelliPATH stainer.
We have tried:
- Trypsine 37C 15 min / Trypsine RT 30 min
- Trypsine (1:2 and 1:3)
- With Decloak