RE: [Histonet] CD31 problewms

2013-02-14 Thread Connolly, Brett M
inal Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 14, 2013 12:27 PM To: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD31 problewms If you have not changed

Re: [Histonet] CD31 problewms

2013-02-14 Thread Rene J Buesa
J.   From: "Jo-Ann Bader, Ms." To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 14, 2013 11:49 AM Subject: [Histonet] CD31 problewms We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale.

[Histonet] CD31 problewms

2013-02-14 Thread Jo-Ann Bader, Ms.
We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With Decloak