RE: [Histonet] COX staining in frosen muscles

2009-09-28 Thread Florence Leomiti
, 2009 12:41 PM To: Florence Leomiti Subject: RE: [Histonet] COX staining in frosen muscles Hello, Florence I work in a research facility and we are beginning to stain frozen rat muscle. I will attempt to stain for COX and would also love to have a copy of your protocol if it isn't asking too

RE: [Histonet] COX staining in frosen muscles

2009-09-28 Thread Florence Leomiti
- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Monday, September 28, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] COX staining in frosen muscles Dear Colleagues. Please help. I

[Histonet] COX staining in frosen muscles

2009-09-28 Thread Galina Deyneko
Dear Colleagues. Please help. I have been asking to perform staining for  COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very  familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the other