Hi everyone, Someone in the lab wishes to look at cultured cells in paraffin embedded cell blocks. These cells grow mostly in non-adherent clumps or cell bodies, which need to be preserved. Sections will be stained by IHC. I've been looking into the matter, and it seems there is not really a single straightforward method for this (as is always the case in histoland, apparently).
I've been thinking to try fixing the cells in either 10% alcoholic formalin for 12-24 hours, or zinc-formalin. Then embed in agarose, fix some more with buffered formaldehyde and process. - What are your thoughts on the fixative of choice? - Should I process slowly, manually; or is a 'standard' ON processor protocol adequate (I don't know the exact timings, but I think it's a twelve hour protocol on a caroussel type processor). - Would Histogel have any added benefit over agarose? Thank you, Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
