Hi fellow Histo people!
I am setting up a new histology lab and I am needing to find inventive ways
to obtain controls for IHC and Special stains. I have lots of normal tissue
but I am in need of melanoma, P16 positive, fungus, positive p63, and
positive p504. If anyone would be interested in help
:47 PM
To: 'Histonet (histonet@lists.utsouthwestern.edu)'
Subject: [Histonet] Controls
STOP, THINK, READ. This is an external email. Exercise extra caution responding
to it, opening attachments and following links.
Good afternoon!
Could any of you tell me off hand how old patient tissue has to
Good afternoon!
Could any of you tell me off hand how old patient tissue has to be before you
can use it as a control? My lab has only been operating since February. We run
quite a few Alcian Blue PAS stains a week, and my last control is getting
sparse. I have some beautiful patient tissue tha
Hello Garrey,
Curious myself, CAP contact info seems to be greyed out on website unless I
officially log in and for now my concerns are with the Washington State Science
and Engineering Fair for K-12 and golf game.
(1) There are at least two phrases in the ANP.21450 which could be parsed out
Center Clinic
> 8333 North Davis Highway
> Pensacola, FL 32514
> Phone 850.474.8581
> Fax 850.474.8584
>
> -Original Message-
> From: Garreyf
> To: "Ronda Mire"
> Cc: histonet@lists.utsouthwestern.edu, "Baker, Michael"
>
> Date: 03/05
ubject: RE: [Histonet] Controls:
Do you mean using Slim Jims right out of the package, as is, for Gram controls?
What is in those things - sounds like a food safety issue!
-Nancy Stedman
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
James Watson
Sent: Thursday, March 05, 2015 1:01 PM
To: 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all
the labs at AFIP.
James Watson HT ASCP
GNF Genomics I
histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
> Sent: Thursday, March 05, 2015 7:21 AM
> To: Jb; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Controls:
>
> We have successfully used hamburg
: Thursday, March 05, 2015 7:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as GMS
controls go, we ran across a post from someone smearing cream cheese onto lung
tissue and letting it sit for a
Cc: histonet@lists.utsouthwestern.edu, "Baker, Michael"
Date: 03/05/2015 11:10
Subject: Re: [Histonet] Controls
I am in need of both a gram and fungal control and will have to try the slim
Jim, hamburger and hotdog tricks. Thanks. If they work I will post
A link to pictures.
Sent f
04, 2015 1:05 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Controls:
>
> Off the wall question, I have been told that slim jims (pepperoni stick) at
> the gas station can be processed and used as good gram controls. Has anyone
> done th
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:
Off the wall question, I have been told that slim j
sts.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire
>Sent: Thursday, March 05, 2015 8:59 AM
>To: Baker, Michael
>Cc: histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Controls
>
>Slim Jim will work as a control for gram sta
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire
Sent: Thursday, March 05, 2015 8:59 AM
To: Baker, Michael
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls
Slim Jim will work as a control for gram stain. Can you believe people eat
this crap?
> On Mar 5, 2
Pathology
>>
>>> On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu
>>> wrote:
>>>
>>> ------
>>>
>>> Message: 9
>>> Date: Wed, 4 Mar 2015 14:04:52 -0700
>>> From: Jb
>>> Subj
Pathology
>
>> On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
>>
>> --
>>
>> Message: 9
>> Date: Wed, 4 Mar 2015 14:04:52 -0700
>> From: Jb
>> Subject: [Histonet] Controls:
>>
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
There was actually an article written in The Journal of Histotechnology a
few years back on this and it was, I f I recall, using a hot dog to create
the gram control. Might be in the archives on nsh.org.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Sent: Thursday, March 05, 2015 9:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as GMS
controls go, we ran across a post from someone smearing cream cheese onto lung
tissue and letting it sit
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:
Off the wall question, I have been told that slim jims (pepperoni stick) at the
gas station can be processed and used as good gram con
Off the wall question, I have been told that slim jims (pepperoni stick) at the
gas station can be processed and used as good gram controls. Has anyone done
this and do they work for GMS also?
Thank you,
Sent from my iPhone
___
Histonet mailing list
H
Help! Running low on CMV and AFB positive control blocks. Looking into buying
precut slides but the price is steep. Any suggestion ? Which company has the
best price.
The information in this
Is a candid control one that's taken when the fungus doesn't know you're
sampling it?
Sincerely?
Jay A Lundgren,
M.S., (HTL) ASCP
On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L <
dorothy.
Does anyone out in histoland have a Candid control for fungus that you could
spare? We are very much in need and would appreciate the help and see what we
could possibly trade for.
Much thanks and have a great week-end all!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254
What are people using to control direct IF?
We use frozen tonsil for immunoglobulins and light chains, but what about
fibrinogen, albumin, C1q, C3c and C4c?
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
We are in need of Aspergillus and Amyloid controls. Does anyone on the
histonet have any suggestions, or overabundance that they could share with us?
Thanks,
Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport,
TN 423 224-5793 fax 423 224-5349
We place control tissue on EACH slide for IHC testing - even if multiple blocks
from same case.
Nancy Schmitt HT, MLT(ASCP)
United Clinical Laboratories
-
Message: 12
Date: Wed, 20 Jul 2011 13:18:52 +
From: "Rathborne, Toni"
Subject: RE:
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls wi
In our Lab we put a control on every slide. We keep multiple controls cut
and stored in refrigerator. Most are multi- tissue controls. We use the
Ventana detection kits and it is not cost efficient to use 2 uses of the
detection kit per antibody. Of course there are times that this will not
work,
e slides?
>
> -Original Message-
> From: Richard Cartun [mailto:rcar...@harthosp.org]
> Sent: Tuesday, July 19, 2011 6:40 PM
> To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
> Subject: Re: [Histonet] Controls with patient specimen on same slide
>
> We do not put
...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks
for a case, or jus
f Of
Rathborne, Toni
Sent: Wednesday, July 20, 2011 8:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EAC
It is the most effective and economical way to run your IHC's.
We have been doing it that way for as long as I have been in histology.
Thanks,
Tim
- Original Message -
From:
To:
Sent: Tuesday, July 19, 2011 6:04 PM
Subject: Histonet Digest, Vol 92, Issue 25
Hi,
> I'm interested in kno
40 PM
To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate biopsies)
that we use fo
outhwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, July 19, 2011 18:40
To: histonet@lists.utsouthwestern.edu; Toni Rathborne
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many o
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate biopsies)
that we use for IHC testing are cut in our Histology Laboratory as part of a
part-type slide protocol. For example, we cut 7 slides, 2 sections o
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Tuesday, July 19, 2011 2:43 PM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls with patient specimen on same slide
Hi Toni,
We put the control on the same slide and after it's stained and
Sent: Tuesday, July 19, 2011 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls with patient specimen on same slide
Hi,
I'm interested in knowing how many of you are performing ihc with the
control tissue and the patient tissue on the same slide. I have seen
sli
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a "C" or "P" written on it.
Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni <
trathbo...@somerset-healthc
Hi,
I'm interested in knowing how many of you are performing ihc with the control
tissue and the patient tissue on the same slide. I have seen slides available
which have designated areas for each tissue to be placed so there will not be
any confusion. If you're doing it, have you encountered a
This response is late due to the overwhelming time commitments of NCAA
Basketball Tournaments.
The only "multi-tissue" control we use is for HER2 protein overexpression. In
my opinion, "multi-tissue" controls are completely unnecessary for every-day
use for the majority of IHC tests that we do
Hello everyone,
I have an issue that I would like some help with. We are currently running
SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the
regulations being so strict regarding the processing times of breast
specimens, how are you all dealing with that when it comes t
Hello,
It seems we go back and forth between multi-tissue control blocks and
single tissue control blocks when it comes to immunostaining.
In the past we used single blocks and still continue to use some for the
IPs that are rarely used. Then, switched to creating multi-tissue
blocks for eas
Does anyone out there in Histoland have a control block for MDM2 for
liposarcoma that they would be willing to share? We don't get very many
liposarcomas so control blocks are a problem. Any help is greatly appreciated.
Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Car
I just wanted to thank all you fellow histonetters who answered my plea for
H. Pylori and / or GMS control blocks. They are great! This trading of
controls is so much nicer than having to buy them. Thanks again for your
help!
Deanne Knutson
___
Hi
I second that. Marsha is the loveliest person I know.
- Original Message -
From: "Denise Piontek"
To:
Sent: Wednesday, April 08, 2009 8:49 PM
Subject: RE: [Histonet] Controls needed!
Newcomer Supply has been a reliable control source, when necessary the have
"cu
Newcomer Supply has been a reliable control source, when necessary the have
"customized orders".
> Date: Wed, 8 Apr 2009 07:14:21 -0500
> From: jennifer.l.hofec...@vanderbilt.edu
> To: dknut...@primecare.org; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet]
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hofecker, Jennifer L
Sent: 08 April 2009 13:14
To: Knutson, Deanne; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls needed!
Hi Deanne,
Have you checked with the NSH
...@primecare.org]
Sent: Tuesday, April 07, 2009 3:06 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Controls needed!
We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We
have
good GRAM control b
We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We have
good GRAM control blocks that we would be happy to exchange. Please let me
know if you can help us out. Thank you!
Deanne Knutson
Anatomic Patho
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