[Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Nancy Schmitt
[Histonet] Controls with patient specimen on same slide To: "'Richard Cartun'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3ad061fe740d464fac7bf6b5cfb75707031...@smcmail01.somerset-healthcare.com> Content-Type: text/pla

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Tom McNemar
] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate

[Histonet] Controls with patient specimen on same slide

2011-07-20 Thread SHargrove
In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work,

Re: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Mark Tarango
e slides? > > -Original Message- > From: Richard Cartun [mailto:rcar...@harthosp.org] > Sent: Tuesday, July 19, 2011 6:40 PM > To: histonet@lists.utsouthwestern.edu; Rathborne, Toni > Subject: Re: [Histonet] Controls with patient specimen on same slide > > We do not put

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Settembre, Dana
...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question.

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Kuhnla, Melissa
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or jus

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Sebree Linda A
f Of Rathborne, Toni Sent: Wednesday, July 20, 2011 8:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EAC

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Tim Higgins
It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim - Original Message - From: To: Sent: Tuesday, July 19, 2011 6:04 PM Subject: Histonet Digest, Vol 92, Issue 25 Hi, > I'm interested in kno

RE: [Histonet] Controls with patient specimen on same slide

2011-07-20 Thread Rathborne, Toni
40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use fo

RE: [Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Weems, Joyce
outhwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 19, 2011 18:40 To: histonet@lists.utsouthwestern.edu; Toni Rathborne Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many o

Re: [Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Richard Cartun
We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections o

RE: [Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Setlak, Lisa
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, July 19, 2011 2:43 PM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Controls with patient specimen on same slide Hi Toni, We put the control on the same slide and after it's stained and

RE: [Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Sebree Linda A
Sent: Tuesday, July 19, 2011 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen sli

Re: [Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Mark Tarango
Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a "C" or "P" written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni < trathbo...@somerset-healthc

[Histonet] Controls with patient specimen on same slide

2011-07-19 Thread Rathborne, Toni
Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered a