[Histonet] Controls with patient specimen on same slide
To: "'Richard Cartun'" ,
"histonet@lists.utsouthwestern.edu"
Message-ID:
<3ad061fe740d464fac7bf6b5cfb75707031...@smcmail01.somerset-healthcare.com>
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]
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate
In our Lab we put a control on every slide. We keep multiple controls cut
and stored in refrigerator. Most are multi- tissue controls. We use the
Ventana detection kits and it is not cost efficient to use 2 uses of the
detection kit per antibody. Of course there are times that this will not
work,
e slides?
>
> -Original Message-
> From: Richard Cartun [mailto:rcar...@harthosp.org]
> Sent: Tuesday, July 19, 2011 6:40 PM
> To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
> Subject: Re: [Histonet] Controls with patient specimen on same slide
>
> We do not put
...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks
for a case, or jus
f Of
Rathborne, Toni
Sent: Wednesday, July 20, 2011 8:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EAC
It is the most effective and economical way to run your IHC's.
We have been doing it that way for as long as I have been in histology.
Thanks,
Tim
- Original Message -
From:
To:
Sent: Tuesday, July 19, 2011 6:04 PM
Subject: Histonet Digest, Vol 92, Issue 25
Hi,
> I'm interested in kno
40 PM
To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate biopsies)
that we use fo
outhwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, July 19, 2011 18:40
To: histonet@lists.utsouthwestern.edu; Toni Rathborne
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many o
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate biopsies)
that we use for IHC testing are cut in our Histology Laboratory as part of a
part-type slide protocol. For example, we cut 7 slides, 2 sections o
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Tuesday, July 19, 2011 2:43 PM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls with patient specimen on same slide
Hi Toni,
We put the control on the same slide and after it's stained and
Sent: Tuesday, July 19, 2011 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls with patient specimen on same slide
Hi,
I'm interested in knowing how many of you are performing ihc with the
control tissue and the patient tissue on the same slide. I have seen
sli
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a "C" or "P" written on it.
Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni <
trathbo...@somerset-healthc
Hi,
I'm interested in knowing how many of you are performing ihc with the control
tissue and the patient tissue on the same slide. I have seen slides available
which have designated areas for each tissue to be placed so there will not be
any confusion. If you're doing it, have you encountered a
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