Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Maxim Peshkov
hanol. Maxim Peshkov, Russia, Taganrog. Original message--- From: "Johnson, Kevin" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, February 8, 2013 1:00 PM Subject: [Histonet] Could these samples have been saved? Dear all, During an overnight tissue processing

Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Rene J Buesa
You are right. You should have tried to rehydrate the tissues, especially mouse that is very lean in itself. You cannot unmummify them now. René J. From: "Johnson, Kevin" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, February 8, 2013 1:00 PM Subject:

RE: [Histonet] Could these samples have been saved?

2013-02-08 Thread joelle weaver
are made this pretty easy to do. Does anyone know differently? Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 8 Feb 2013 13:22:15 -0500 > From: kgrob...@rci.rutgers.edu > To: kjohn...@med.miami.edu > Subject: Re: [Histonet] Could these samples have been saved? > CC: histonet@li

Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread kgrobert
I don't know about your samples now, but for future reference, you might find this interesting: http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharma

Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Roger Heyna
We microwave process our tissues, and we've had tissues fall out of the rack after the dehydration phases and air dry in the open retort for hours. We did not continue with processing; we added them to formalin for eight hours (which could be overkill) and then started the processing from the be

[Histonet] Could these samples have been saved?

2013-02-08 Thread Johnson, Kevin
Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the