Re: [Histonet] Coverslipping mystery

2015-07-13 Thread Caroline Miller
; Adam > > -Original Message- > From: Caroline Miller [mailto:mi...@3scan.com] > Sent: 11 July 2015 15:18 > To: John Kiernan > Cc: Adam Boanas; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Coverslipping mystery > > I really like DPX, although funnil

Re: [Histonet] Coverslipping mystery

2015-07-13 Thread Bernice Frederick
] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always

Re: [Histonet] Coverslipping mystery

2015-07-13 Thread Adam Boanas
edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and

Re: [Histonet] Coverslipping mystery

2015-07-11 Thread Caroline Miller
I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a

Re: [Histonet] Coverslipping mystery

2015-07-10 Thread John Kiernan
DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The

Re: [Histonet] Coverslipping mystery

2015-07-09 Thread Jennifer MacDonald
What type of clearing agent are you using? The aliphatic hydrocarbons are not compatible with all mounting media. From: Adam Boanas To: "histonet@lists.utsouthwestern.edu" Date: 07/09/2015 06:16 AM Subject: [Histonet] Coverslipping mystery Hello, We are having

Re: [Histonet] Coverslipping mystery

2015-07-09 Thread Goins, Tresa
] Coverslipping mystery Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by

Re: [Histonet] Coverslipping mystery

2015-07-09 Thread Rene J Buesa
Adam: Just guessing, but I think the "mystery" is caused by how fluid the DPX is. Guessing again, but it probably is more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.René On Thursday, July 9, 2015 9:40 AM, Adam Boanas wro

Re: [Histonet] Coverslipping mystery

2015-07-09 Thread Simmons, Christopher
- From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, July 09, 2015 9:47 AM To: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery Adam: Just guessing, but I think the "mystery" is caused by how fluid the DPX is. Guessing again, but it p

[Histonet] Coverslipping mystery

2015-07-09 Thread Adam Boanas
Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that