I was asking about the cutting head temperature because the Leica CM3050
literature says the cutting head can be adjusted down to -50c separately from
the chamber temperature. Does anyone out there use this unit? It seems to be
very popular with the research community. Do you ever adjust the te
Hello,
We need to cut clean and consistent 50 micron sections of OCT embedded
tissue on our leica cryostat (CM-1850). Does anyone on the list have
any tips or tricks for doing this when compared to cutting the usual
<10 micron sections that we usually deal with in our lab.
--
Regards,
Francis
It may help to let your block equilibrate for about 20 minutes in the
cryostat. I've found blocks do not section well if you start sectioning
right away, especially snap frozen ones. Also, do you embed in OCT/sucrose?
A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever
embed
I am new to using a cryostat and am having some issues with my tissue.
1.Every slice seems to shatter and fragment. When I have used a
microtome it was because the tissue was too cold.
2. The tissue itself was flash frozen and so was not fixed and the tissue
did not go through t
