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Today's Topics:
1. Re:Endogenous biotin blocking (Hobbs, Carl)
2. Marginated Staining on IHC (Katelin Lester)
3. Re: H&E preparation (Rene J Buesa)
4. Book specific for Neuropathology techniques/methods (Sharon Allen)
5. RE: H&E preparation (Tony Henwood)
6. Mast cell staining HELP! (Kim O'Sullivan)
7. RE: H&E preparation (Kemlo Rogerson)
8. RE: H&E preparation (Lynette Pavelich)
9. Ink issues (Amspacher, September)
10. RE: Ink issues (Walzer Susan)
11. RE: Gloves (Bonner, Janet)
12. RE: Ink issues (Smith Wanda)
13. RE: Ink issues (Weems, Joyce)
14. RE: H&E preparation (Weems, Joyce)
15. Robotic Prostatectomy (Phyllis Thaxton)
16. (no subject) (James, Jennifer)
17. Cost to produce an H&E slide (Amy Johnson)
18. Re: Robotic Prostatectomy (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Tue, 1 Sep 2009 19:23:46 +0100
From: "Hobbs, Carl" <carl.ho...@kcl.ac.uk>
Subject: [Histonet] Re:Endogenous biotin blocking
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<11d9615b89c10747b1c985966a63d7ca2991742...@kcl-mail04.kclad.ds.kcl.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Your understanding is correct, imho.
I add a "no primary" control on my stABCpx-DAB run whenever I am testing new
tissues and assess any positivity that could be due to endogenous biotin.
You will quickly build up a knowledge of those tissues that automatically
require biotin - blocking. ( eg liver, kidney)
If you are a clinical/registered Lab, you wil of course be required to buy a
commercial kit.
If you are in a research lab and find that you will need to apply biotin
blocking regularly, you can make up your own avidin/biotin solutions if costs
are a major factor.
I will be interested in other suggestions/approaches.
carl
------------------------------
Message: 2
Date: Tue, 1 Sep 2009 11:47:22 -0700
From: "Katelin Lester" <kate...@cuttingedgehistology.com>
Subject: [Histonet] Marginated Staining on IHC
To: <histonet@lists.utsouthwestern.edu>
Message-ID: <f4175dfdcb414f1ba1f2ad3e3ffef...@front>
Content-Type: text/plain; charset="us-ascii"
Hi histonet,
I am back with a problem I've been having with my IHC slides. We had a PM
recently and had thought that would solve this problem, but as I do more and
more slides, the problem remains. I am seeing marginated staining at the
edge of the tissue on the controls as well as the patient tissue. This
occurs at the top, middle, and bottom of the slides. This occurs with any
antibody. This does not occur with every run, but the more slides I have on
the stainer, the more frequently it occurs. I have contacted Thermo, who
now takes care of our old R.A.S. Microm HMS 710i, but I have further faith
that the histonet can help me. I've seen similar posts in the archives, but
did not see any responses.
Any suggestions are appreciated,
Katelin
Katelin Lester
Cutting Edge Histology Services, LLC
(503) 443-2157
------------------------------
Message: 3
Date: Tue, 1 Sep 2009 12:22:06 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] H&E preparation
To: histonet@lists.utsouthwestern.edu, Aazath Raj <aaz...@hotmail.com>
Message-ID: <960806.99145...@web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=utf-8
The hematoxylin has to be ripen, either naturally (it takes long time) or with
an oxidizer.
René J.
--- On Tue, 9/1/09, Aazath Raj <aaz...@hotmail.com> wrote:
From: Aazath Raj <aaz...@hotmail.com>
Subject: [Histonet] H&E preparation
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 1, 2009, 11:48 AM
Dear All,
     We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does anybody know
how to control a consistency in the strength .
Aazath
Technical Officer
Apollo Hospitals
India
_________________________________________________________________
News, sports, entertainment and fine living…learn the ropes on MSN India
http://in.msn.com_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Tue, 1 Sep 2009 16:14:18 -0500
From: "Sharon Allen" <sal...@exchange.hsc.mb.ca>
Subject: [Histonet] Book specific for Neuropathology
techniques/methods
To: <histo...@pathology.swmed.edu>
Message-ID:
<bb6adcd4b7abb045a09a7634ac15cc610bec8...@hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="us-ascii"
Hi,
Can anyone on the Histonet recommend a good book of Neuropathology
techniques/methods for use in a Neuropathology Lab. Need more up to
date reference material.
Thanks
Sharon Allen
Neuropathology Lab
HSC - Wpg
sal...@hsc.mb.ca
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Message: 5
Date: Wed, 2 Sep 2009 10:21:20 +1000
From: "Tony Henwood" <antho...@chw.edu.au>
Subject: RE: [Histonet] H&E preparation
To: "Aazath Raj" <aaz...@hotmail.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID: <b9eaf61856077f47bf9be2f89afc555206853...@hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Aazath,
Several possibles:
It can come down to the ripening time of the haematoxylin.
Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!)
mercury oxide, added) will tend to stain strongly initially but tend to
over-oxidise giving weak or brown nuclear staining.
Heating the solution and adding the oxidiser will cause the same thing.
Less oxidiser will give you a solution with a longer self life but
weaker staining.
If the pH is too low, staining will be weaker, but more specific.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: Wednesday, 2 September 2009 1:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation
Dear All,
We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .
Aazath
Technical Officer
Apollo Hospitals
India
_________________________________________________________________
News, sports, entertainment and fine living...learn the ropes on MSN
India http://in.msn.com_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 6
Date: Wed, 02 Sep 2009 12:33:52 +1000
From: Kim O'Sullivan <kim.osulli...@med.monash.edu.au>
Subject: [Histonet] Mast cell staining HELP!
To: histonet@lists.utsouthwestern.edu
Message-ID: <130.194.114.97.1251858...@my.monash.edu.au>
Content-Type: text/plain; charset=UTF-8
Hi,
Can anyone out there recommend the best way to stain mouse mast cells in the
kidney(toluidine blue/or berberine sulfate) with a protocol that produces
cconsistent results?
Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys.
Does anyone know of a company that supplies antibodies which will work on any
of these fixatives,for the staining of the IgE receptor, mast cell trytptase
and chymase.
Any advice would be appreciated!
Kim O'Sullivan
Monash University
Melbourne
Australia
------------------------------
Message: 7
Date: Wed, 2 Sep 2009 08:53:46 +0100
From: "Kemlo Rogerson" <kemlo.roger...@waht.swest.nhs.uk>
Subject: RE: [Histonet] H&E preparation
To: "Aazath Raj" <aaz...@hotmail.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID:
<86ade4eb583ce64799a9924684a0fbbf07727...@wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Ripening in the sun (we do get some in the UK) used to give Haematoxylin
that was stable; if you forgot to make any up then you hastely added
oxidising agent to a new batch or 'jumped started' a batch that was as
yet still immature. That batch then never seemed to last as long as you
got muddy staining. They looked quite pretty, lines of 5 litres flasks
with their contents ripening in the sun with cotton wool balls sticking
out of the top, next to the cigarette ashtrays. How things have
changed..............
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: 01 September 2009 16:49
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation
Dear All,
We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .
Aazath
Technical Officer
Apollo Hospitals
India
_________________________________________________________________
News, sports, entertainment and fine living...learn the ropes on MSN
India http://in.msn.com_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 02 Sep 2009 07:01:39 -0400
From: "Lynette Pavelich" <lpave...@hurleymc.com>
Subject: RE: [Histonet] H&E preparation
To: <aaz...@hotmail.com>,<histonet@lists.utsouthwestern.edu>,
<kemlo.roger...@waht.swest.nhs.uk>
Message-ID: <4a9e1853020000ee0002c...@smtp-gw.hurleymc.com>
Content-Type: text/plain; charset=US-ASCII
Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric
oxide) too soon when it was still bubbling and having a volcano of
hematoxylin?!!! LOLj
Was remembering tho', that every day, before using, we would "top off"
(add...oh, about 30ml) of fresh to the filtered used batch. This really
kept the quality good. Worked for 30 yrs until we started purchasing
it. Gone are the morning coffee and donuts sitting next to our
microtomes too!! Sigh................ Ya, ya....I know!!!
"Kemlo Rogerson" <kemlo.roger...@waht.swest.nhs.uk> 09/02/09 3:53 AM
Ripening in the sun (we do get some in the UK) used to give Haematoxylin
that was stable; if you forgot to make any up then you hastely added
oxidising agent to a new batch or 'jumped started' a batch that was as
yet still immature. That batch then never seemed to last as long as you
got muddy staining. They looked quite pretty, lines of 5 litres flasks
with their contents ripening in the sun with cotton wool balls sticking
out of the top, next to the cigarette ashtrays. How things have
changed..............
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: 01 September 2009 16:49
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation
Dear All,
We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .
Aazath
Technical Officer
Apollo Hospitals
India
_________________________________________________________________
News, sports, entertainment and fine living...learn the ropes on MSN
India http://in.msn.com_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 2 Sep 2009 07:02:30 -0400
From: "Amspacher, September" <september.amspac...@bassett.org>
Subject: [Histonet] Ink issues
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID: <71a87f8feffb024db88b064543c7f24b13283...@ex2.bassett.org>
Content-Type: text/plain; charset="iso-8859-1"
Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and
blue that we are using are fine we have tried several different companies and even use a "ink stay"
acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked
into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of
this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am
hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to
"fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
September Amspacher HT(ASCP)
Technical Specialist- Histology Department
Laboratory Chemical Hygiene Officer
Bassett Healthcare
Cooperstown, New York
------------------------------
Message: 10
Date: Wed, 2 Sep 2009 07:18:22 -0500
From: Walzer Susan <susan.wal...@hcahealthcare.com>
Subject: [Histonet] RE: Ink issues
To: "Amspacher, September" <september.amspac...@bassett.org>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<4bf03f5404ebde409af9232da74b9ded2ac41fd...@fwdcwpmsgcms09.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
My Docs use acetone.
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher,
September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ink issues
Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and
blue that we are using are fine we have tried several different companies and even use a "ink stay"
acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked
into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of
this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am
hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to
"fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
September Amspacher HT(ASCP)
Technical Specialist- Histology Department
Laboratory Chemical Hygiene Officer
Bassett Healthcare
Cooperstown, New York
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Wed, 2 Sep 2009 09:10:42 -0400
From: "Bonner, Janet" <janet.bon...@flhosp.org>
Subject: RE: [Histonet] Gloves
To: "Merced M Leiker" <lei...@buffalo.edu>, "Mahoney,Janice A"
<janice.maho...@alegent.org>, "Histonet"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<5f31f38c96781a4fbe3196ebc22d47807f2...@fhosxchmb006.adventistcorp.net>
Content-Type: text/plain; charset=iso-8859-1
We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size)
www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand
'like a glove' . They say on the box "not intended to be used as a chemical
barrier", but they do a great job when exposed to Histology chemicals.
Janet
________________________________
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Merced M Leiker
Sent: Mon 8/31/2009 12:43 PM
To: Mahoney,Janice A; 'Histonet'
Subject: Re: [Histonet] Gloves
I would like to know the answer to this one too. I've been using nitrile
gloves, which are rated as having moderate protection against xylene (they
slow down how quickly xylene goes through them?) The brand I use is what's
in our stockroom: Kimberly Clark and they come in a pretty purple. :-)
--On Monday, August 31, 2009 11:15 AM -0500 "Mahoney,Janice A"
<janice.maho...@alegent.org> wrote:
I'd like some advice about gloves. What brand/vendor are people using
that are approved for use with xylene? Thanks,
Jan Mahoney
Omaha
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------------------------------
Message: 12
Date: Wed, 2 Sep 2009 09:07:02 -0500
From: Smith Wanda <wanda.sm...@hcahealthcare.com>
Subject: [Histonet] RE: Ink issues
To: "Amspacher, September" <september.amspac...@bassett.org>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<9e2d36ce2d7cba4a94d9b22e8328a3bafc796...@nadcwpmsgcms03.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc.
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher,
September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ink issues
Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and
blue that we are using are fine we have tried several different companies and even use a "ink stay"
acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked
into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of
this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am
hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to
"fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
September Amspacher HT(ASCP)
Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer
Bassett Healthcare Cooperstown, New York
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 2 Sep 2009 10:09:55 -0400
From: "Weems, Joyce" <jwe...@sjha.org>
Subject: RE: [Histonet] Ink issues
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
<5d64396a0d4a5346bebc759022aaeaa57c2...@itsssxm01v6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We use acetic acid - 3-5% solution (vinegar). j
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Amspacher, September
Sent: Wednesday, September 02, 2009 07:03
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ink issues
Good Morning all- I am having issues getting ink to "stick" to the
tissue surface, the black and blue that we are using are fine we have
tried several different companies and even use a "ink stay" acetic acid
spray on the tissue. My new pathologist has talked about dipping the
tissue after it is inked into Bouins solution, and that they didn't have
any issues with inks where he came from. I have heard of this technique
before, But I was just able to get rid of all of the bounins solutions
in the lab and I am hesitant to bring it back, looking for Ideas and
thought from all, also if you use the Bouins solution to "fix" your
inks- I would love to learn your thoughts about the process.
Thanks-a-million
September Amspacher HT(ASCP)
Technical Specialist- Histology Department Laboratory Chemical Hygiene
Officer Bassett Healthcare Cooperstown, New York
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
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It may contain information that is privileged and
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------------------------------
Message: 14
Date: Wed, 2 Sep 2009 10:14:10 -0400
From: "Weems, Joyce" <jwe...@sjha.org>
Subject: RE: [Histonet] H&E preparation
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
<5d64396a0d4a5346bebc759022aaeaa57c2...@itsssxm01v6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
the good ole days for sure - purple ceilings, purple walls, purple
people.... And cigarette ashes in the trash and food on the counter...
Is it Friday yet?!!!
J:>)
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich
Sent: Wednesday, September 02, 2009 07:02
To: aaz...@hotmail.com; histonet@lists.utsouthwestern.edu;
kemlo.roger...@waht.swest.nhs.uk
Subject: RE: [Histonet] H&E preparation
Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric
oxide) too soon when it was still bubbling and having a volcano of
hematoxylin?!!! LOLj Was remembering tho', that every day, before
using, we would "top off"
(add...oh, about 30ml) of fresh to the filtered used batch. This really
kept the quality good. Worked for 30 yrs until we started purchasing
it. Gone are the morning coffee and donuts sitting next to our
microtomes too!! Sigh................ Ya, ya....I know!!!
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------------------------------
Message: 15
Date: Wed, 2 Sep 2009 08:26:06 -0700 (PDT)
From: Phyllis Thaxton <dch...@yahoo.com>
Subject: [Histonet] Robotic Prostatectomy
To: histonet@lists.utsouthwestern.edu
Message-ID: <21877.6306...@web43503.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
For the first time here, we have Urologists using the robotic method for
removing prostates. The PA always receives them fresh, inks them, then puts the
whole prostate in alcoholic fixative (currently Zfix from Anatech). The next
day they are grossed in and processed. The prostates we have received lately
are raw in the middle and unable to be grossed in, whereas before they were
fine.
Has anyone had similar experiences?
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
------------------------------
Message: 16
Date: Wed, 2 Sep 2009 10:54:28 -0500
From: "James, Jennifer" <jamesjennif...@uams.edu>
Subject: [Histonet] (no subject)
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<22ad9c7b0b022246aef93ecf29bdb0d40b317d6...@mail2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
Does anyone have experience with the Cell Technology Apo-single stranded DNA
antibody? Please let me know if you do.
Thanks!
Jennifer James BS, HT(ASCP)HTL, QIHC, CRS
Research Assistant
Experimental Pathology Laboratory
Winthrop P.Rockefeller Cancer Institute, Room 429
Universtiy of Arkansas for Medical Sciences
4301 West Markham Street, #725
Little Rock, AR 72205
501-686-8265
jamesjennif...@uams.edu<mailto:jamesjennif...@uams.edu>
------------------------------
Message: 17
Date: Wed, 2 Sep 2009 11:11:50 -0500
From: "Amy Johnson" <ajohn...@aipathology.com>
Subject: [Histonet] Cost to produce an H&E slide
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
<704247d5a09d004c9e6b115138d1703a073...@hpserv001.aipathology.local>
Content-Type: text/plain; charset="US-ASCII"
Hello Histonet.....Has anyone ever figured out how much it costs to
produce an H&E slide? Are there any articles out there that would help
to figure this out?
I realize it may be different for each institution but a ball park
figure would be greatly appreciated.
Thanks
Amylin Johnson
------------------------------
Message: 18
Date: Wed, 2 Sep 2009 09:51:25 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Robotic Prostatectomy
To: histonet@lists.utsouthwestern.edu, Phyllis Thaxton
<dch...@yahoo.com>
Message-ID: <909608.59241...@web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
And your problem will not be solved unless the prostates, after being inked,
are cut in half before placing them in the fixative. I do not see any problem
in cutting in half a properly inked prostate to assure proper fixation and
subsequent processing.
René J.
--- On Wed, 9/2/09, Phyllis Thaxton <dch...@yahoo.com> wrote:
From: Phyllis Thaxton <dch...@yahoo.com>
Subject: [Histonet] Robotic Prostatectomy
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, September 2, 2009, 11:26 AM
For the first time here, we have Urologists using the robotic method for
removing prostates. The PA always receives them fresh, inks them, then puts the
whole prostate in alcoholic fixative (currently Zfix from Anatech). The next
day they are grossed in and processed. The prostates we have received lately
are raw in the middle and unable to be grossed in, whereas before they were
fine.
Has anyone had similar experiences?
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
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Histonet mailing list
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------------------------------
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End of Histonet Digest, Vol 70, Issue 2
***************************************
