I do manual IHC-DAB regularly
DAB shouldn't "go off" after freezing unless you have somehow oxidised it
during mixing/aliquoting.
It would then show as a dark brown aliquot
I state this because I still make up my own DAB from dry powder ( have been
doing for > 20yrs)
SIgma D5637
I dissolve, aliqu
Hi everyone,
I am doing free-floating immunohistochemistry with brain slices.
That is only the context. The issue is that I always prepare my DAB
(Hydrochloride sigma powder) aliquot it and store it in -20°C. But
recently, once I thaw it the DAB quality decreases a lot, to almost not
have a
