My brief experience with paraffin and fluorescence (DAPI and EGFP) is that
the paraffin embedding process destroys it.
Regards,
Merced
--On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam
wrote:
Hi,
Just wondering if anyone has use hydroethinide to detect the free
radicals (ROS) in t
Hi,
Just wondering if anyone has use hydroethinide to detect the free radicals
(ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and
parrafin embedding.
I wanted to used the hydroethidine on paraffin sections.
I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF)
given how many people cant even figure out how to properly unsubscribe
from this list, i fear your request might be in vain ;-)
Mark Elliott wrote:
Just a friendly reminder that if you are responding to a question to please
delete out everything but the pertinent information, especially if you
Just a friendly reminder that if you are responding to a question to please
delete out everything but the pertinent information, especially if you are
receiving/responding in Digest mode. If you don't cut out the irrelevant posts
it makes it extremely hard to find the new stuff. Some of the Di
