Re: [Histonet] Disposal of Bouin's Solution

2017-05-13 Thread Tyrone Genade via Histonet
Hello, Regarding disposal of Bouin's solution the following protocol might be helpful/amusing/terrifying: https://phc.amedd.army.mil/PHC%20Resource%20Library/Bouins_fixative_solution_FS%20_37-007-0913.pdf For IHC the argument is that Bouin's doesn't destroy antigens as compared to other fixative

Re: [Histonet] Disposal of Bouin's Solution

2017-05-13 Thread Hobbs, Carl via Histonet
"I agree it is very dangerous.  When I discovered a dried bottle of picric acid someone had donated to the school right after I first came to the university.  I had to call the fire department and the bomb squad came to get it.  They took it to the firing range and blew a huge sized whole in t

Re: [Histonet] Disposal of Bouin's Solution

2017-05-12 Thread Shirley A. Powell via Histonet
:16 PM To: Histonet@lists.utsouthwestern.edu Cc: Bob Richmond Subject: Re: [Histonet] Disposal of Bouin's Solution https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf Two hazards: formaldehyde and picric acid (see attached). While I agree with Dr. Richmond, changing protoco

Re: [Histonet] Disposal of Bouin's Solution

2017-05-12 Thread Hobbs, Carl via Histonet
Not a disposal query. "There are substitutes for it for most purposes." Bob, I am interested in "most" and "substitutes" In what situation does "most" not apply? What substitutes? For example, I read that tissues are fixed in Picric acid-containing Formalin for IHC/IF. Why? What's the logi

Re: [Histonet] Disposal of Bouin's Solution

2017-05-12 Thread Monson, Frederick via Histonet
estern.edu] Sent: Thursday, May 11, 2017 6:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Disposal of Bouin's Solution Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are curr

Re: [Histonet] Disposal of Bouin's Solution

2017-05-11 Thread Bob Richmond via Histonet
Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd li

[Histonet] Disposal of Bouin's Solution

2017-05-10 Thread Lori Jones via Histonet
We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's Solution