This is probably most easily done using immunofluorescence since it works well
on frozen tissue. You could use a streptavidin linked FITC tag for the human
biotinylated Ab, and then use a red tagged anti IgG directed against the
species of the other antibody. (hoescht reagent will stain the nuc
er via Histonet"
To: "gu lang"
Cc: "Histonet@Lists. Edu"
Sent: Saturday, February 20, 2016 8:35:18 AM
Subject: Re: [Histonet] Double stain IHC question
+ to Gudrun's comments.
My addition is that fluorescence labels may work for this application.
Again, dependin
20. Februar 2016 01:54
> An: histonet@lists.utsouthwestern.edu
> Betreff: [Histonet] Double stain IHC question
>
> Hi everyone,
> I have a question in chromogenic double staining. Here is the situation.
> Tissue = human, frozen
> Antibody = same pro
..
Gudrun
-Ursprüngliche Nachricht-
Von: Judi Ford via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Samstag, 20. Februar 2016 01:54
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Double stain IHC question
Hi everyone,
I have a question in chromogenic double staining
Hi everyone,
I have a question in chromogenic double staining. Here is the situation.
Tissue = human, frozen
Antibody = same protein (A)
1. Commercial antibody of A
2. Homegrown antibody of A, human, biotinylated
Question: can you stain both versions o
