Thanks for all the responses, I will keep the eosin in alcohol for now.
Don't worry, I will follow all CAP rules.
Thanks again.
--
*Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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Gareth Davis asked about marking small GI specimens with dye when grossing
them.
I've used safranin O - the solution the microbiologists use in the Gram
stain. If you walk down the hall to the micro lab you can get a small
amount to try out.
Do not use eosin for this purpose. Eosin's brilliant fl
@lists.utsouthwestern.edu
Subject: [Histonet] Eosin on processor for biopsies
Gareth,
reguarding the eosin biopsy post on the histo net. Things you might want to
considered looking at, I cannot say for sure because I don't know your
processing solutions/schedule:
Most Eosins are alcohol soluable, by pu
Gareth,
reguarding the eosin biopsy post on the histo net. Things you might want to
considered looking at, I cannot say for sure because I don't know your
processing solutions/schedule:
Most Eosins are alcohol soluable, by putting the eosin in your formalin most of
it will wash out during proc
For those of you that use Eosin in the processing of tissue. Can you please
tell me what kind of Eosin mixture you use and how much?
Thanks
Diane
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Elizabeth M. Cameron, HT(ASCP), QIHCCM. Lead Histologist at Mid Coast
Hospital in Brunswick, Maine asks:
>>I have been staining fish tissues fixed in Davidson's fixative with H&E,
and the researcher would like the eosin to be more intense. Our standard
protocol works well for our own tissue, but t
ology Laboratories
Institute of Aquaculture
University of Stirling
Stirling, FK9 4LA
Scotland
UK
-Original Message-
From: Cameron, Elizabeth via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: 30 August 2016 15:25
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin
You might try some combination of eosin and biebrich scarlet and/or
phloxine.
Adding a little acetic acid might help.
http://www.stainsfile.info/StainsFile/downloads/eosin.pdf
I am quite fond of Biebrich Scarlet and like way it stains brain and
heart particularly.
Biebrich is much more red
016 07:29 AM
Subject:[Histonet] Eosin
Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the
researcher would like the eosin to be more intense. Our standard protocol
works well for our own tissue, but the fish look much more washed out. I
am using alcoholic eosin
Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the
researcher would like the eosin to be more intense. Our standard protocol
works well for our own tissue, but the fish look much more washed out. I am
using alcoholic eosin Y, have tried both water and alcohol before and
Hello Rebecca,
Reguarding the following message:
"I had a biopsy today that was nearly impossible to see on the sponges during
embedding or in the block. I've heard mention of marking these with eosin to
make them easier to see. Has anyone done this? Or do you use some other type
of markin
Jim Vickroy, Histology Manager, Springfield Clinic, Springfield, Illinois
asks:
>>Someone told me that you could not use eosin on the new Peloris II. Many
of us use eosin to color small biopsies during the process run. We have it
in one of the 95% ETOHs. Can someone explain why this can't be used
- Room SSS-024
MailDrop: Geology-Astronomy
750 South Church Street
West Chester, PA, 19383
610-738-0437
fmon...@wcupa.edu
-Original Message-
From: Kienitz, Kari [mailto:kkien...@orclinic.com]
Sent: Monday, July 13, 2015 11:21 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re
“little Eosin in the sections” problem.. not just the first rack. Again
any and all suggestions are welcomed!! Thanks again Rene and Kari for
replying. Valerie From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthw
just the first rack.
Again any and all suggestions are welcomed!!
Thanks again Rene and Kari for replying.
Valerie
From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin
It is
is message and its attachments from
your computer system. Thank you
From: Hannen, Valerie [valerie.han...@parrishmed.com]
Sent: Monday, July 13, 2015 8:05 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin
Good morning,
I once again am dea
It is difficult to advise you anything without knowing what is your PT's
complaint. What he does complain about?
René
On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie"
wrote:
Good morning,
I once again am dealing with a my picky Pathologist!! About a year ago, he
started to c
Good morning,
I once again am dealing with a my picky Pathologist!! About a year ago, he
started to complain about not having enough Eosin on his sections( first
morning rack on Monday).. I went from changing the Eosin on Friday and stirring
it on Monday to totally changing it on Monday...it
Dissolve it in a buffer (eg acetic acid-sodium acetate) at or near pH 4.5.
John Kiernan
London, Canada
= = =
On 23/04/15, Pablo Sanchez-Quinteiro wrote:
> Listers, Could you tell me the best way to adjut the eosin pH to 4-5?
>
> Thanks in advance
>
> Pablo
>
>
Listers, Could you tell me the best way to adjut the eosin pH to 4-5?
Thanks in advance
Pablo
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Hi every one
Any one know how to get 3 shades of eosin stain. I am using alcoholic eosin.
Thanks
Manahil Elbireir
HTL/ ASCP
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, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an i
Hi Histonet,
Eosin bleaching is usually due to the alcohols that are used after the eosin,
if you are in a humid area, the alcohols can actually absorb water from the
humidity in the air. This may mean a change of how often you rotate or change
the alcohols, possibly more often than you did
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson';
'histo...@listsutsouthwestern.edu'
Subject: RE: [Histonet] Eos
Subject: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an issue when using fresh alcohols. Does anyone have any other
ideas
ssage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Friday, June 20, 2014 8:15 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin Leaching
We haven't changed our
x27;; 'histonet@lists.utsouthwestern.edu'
> Subject: RE: [Histonet] Eosin Leaching
>
> Have you changed your coverslipping mountant?
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On
...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Have you changed your coverslipping mountant?
-Original Message-
Hello all,
We’re having a problem with our eosin bleeding out of the sections. I’ve read
that this can be caused by not dehydrating adequately after eosin, but we’re
still having an issue when using fresh alcohols. Does anyone have any other
ideas as to the cause of this problem?
Thanks in adv
: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an issue when using fresh alcohols. Does anyone have any other
ideas as to th
esday, September 3, 2013 1:09 PM
Subject: [Histonet] Eosin problem
Hi All,'
Hoping someone can give me some insight on if this happens/ happened to them.
We change all of our reagents on the H&E stainer on Friday( which means the
reagents sit idle over the weekend.) We rarely have to co
Hi All,'
Hoping someone can give me some insight on if this happens/ happened to them.
We change all of our reagents on the H&E stainer on Friday( which means the
reagents sit idle over the weekend.) We rarely have to come in on the weekends
due to having to embed/cut/stain tissues. However,
...@lists.utsouthwestern.edu] Im Auftrag von kristen
arvidson
Gesendet: Freitag, 12. Juli 2013 19:25
An: histonet
Betreff: [Histonet] Eosin Staining Issues
Hello All!
I am having some bizzare staining issues. The eosin seems to be washing
out?? The slides look "sun bleached" according to the Pa
Hello All!
I am having some bizzare staining issues. The eosin seems to be washing out??
The slides look "sun bleached" according to the Pathologists. The problem is
that it is only happening on occasion. One rack stains fine and the next one
has a problem. It even appears to be happen
Dear All,
Please can I ask what everyone uses as a standard Eosin recipe? The new
pathologist in our department has asked for the stain to be darker, 'like the
hospital does it'. I work on the research side of a vet school, so I'm not sure
what histology departments in hospitals normally use.
Eosin on processor can be done with dirty eosin waste saved from the stainer;
adding eosin powder sounds like a mess.
Especially helpful for orienting small samples with a sidedness, skin GI GU
oral, cornea
No common down sides for routine HE, PAS, Trichrome, or IHC even with Red
chromagens.
We
om]
Sent: Wednesday, November 28, 2012 7:54 AM
To: Histonet
Subject: [Histonet] eosin in the processor
Hi Everyone,
Years ago, my lab used to put eosin in the processor to lightly tint the
smaller mouse tissues. I can't remember which station we put it in (I think it
was the 2nd 100% ethanol). Al
Eosin won't interfere with binding of antibodies, but eosin is
fluorescent. You might want to consider that.
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Wednesday, November 28, 2012 5:55 AM
To: Histonet
Subject: [Histonet] eosin in the processor
Hi Everyone,
Years ago, my lab used to put eosin in the processor to lightly tint the
smaller mouse tissues. I can't remember
.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Kim Merriam
[kmerriam2...@yahoo.com]
Sent: Wednesday, November 28, 2012 7:54 AM
To: Histonet
Subject: [Histonet] eosin in the processor
Hi Everyone,
Years ago, my lab used to put eosin in the processor to lightly tint the
smaller mou
Hi Everyone,
Years ago, my lab used to put eosin in the processor to lightly tint the
smaller mouse tissues. I can't remember which station we put it in (I think it
was the 2nd 100% ethanol). Also, back then my lab didn't do any IHC; will the
eosin affect any IHC that might be done (I am gues
I know the original Peloris states that eosin should not be used in the
alcohols It does say you can put it in the formalins.
What are you all doing out there to mark your small needle bx and gi bx's? We
prefer methods where we add to the reagents on the processor.
We have tried just formal
staining.
-Original message-
From: "Hannen, Valerie" valerie.han...@parrishmed.com
Date: Thu, 09 Aug 2012 09:23:26 -0500
To: histonet histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin
> Hi Folks...I am hoping you all give me a little help. Our
Pathologists are
Add a few drops of glacial acetic acid.
Geoff
On 8/9/2012 11:23 AM, Hannen, Valerie wrote:
Hi Folks...I am hoping you all give me a little help. Our Pathologists are
complaining about our Eosin on the H&E's being weak. The funny thing is, is
that it can go one
day to the next...one day it l
What clearing agent are you using?
If it is a xylene substitute then you might want to rotate or change the
alcohols after eosin after a few racks of slides.
We use ProPar on our stainer and rotate our alcohols after eosin after
every 3 racks that have gone through.
On Thu, Aug 9, 2012 at 11:2
Hi Folks...I am hoping you all give me a little help. Our Pathologists are
complaining about our Eosin on the H&E's being weak. The funny thing is, is
that it can go one
day to the next...one day it looks great...the next it is weak!!
I have already done some experimenting with...1) time tissu
So I have always used Eosin-Y stain...can someone tell me which is
better...alcohol or phloxine??
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
__
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson
Sent: Wednesday, June 20, 2012 10:32 AM
To: histonet@lists.utsouthwestern.edu; Carol Freeman
Subject: Re: [Histonet] Eosin staining for small biopsies
We use safranin (used in Microbiology as
We use safranin (used in Microbiology as a counterstain) on our small
biopsies. We apply a small drop during grossing. It does not affect staining of
H&E, IHC or ISH. We like this because it is an intense red that doesn't leach
out in the alcohols of processor.
Cindi Robinson HT(ASCP)
Mercy
Good Morning all, Happy Wednesday!
I have a question and I am not finding much on my google search...First
does anyone have any papers written or studies done on the use of Eosin
to stain small biopsies or the use of eosin on the tissue processor in
the last alcohol?? I have read snippets on eo
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections
Hi,
In our lab we are having trouble with the eosin bleeding off the section
after coverslipping. We are performing a rapid H&E stain on frozen
sections. Any advice would be greatly appreci
100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 11:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet
Hi,
In our lab we are having trouble with the eosin bleeding off the section
after coverslipping. We are performing a rapid H&E stain on frozen
sections. Any advice would be greatly appreciated!
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Robin this is the protocol we use to showing new bone old bone.
Procedure for Eosin Y 0.6 % For Staining Bone Specimen
Chemicals
EosinY, Sigma E-4382,
100% Ethanol,
Phloxine B (Sigma P-4030)
Orange G(sodium salt-Sigma O-1625)
Preparation:
Stock 0.6% Eosin
Add 6g of eosin to 900ml 100% Ethanol and1
Happy Monday Everyone!
I guess I am having a old persons momment! I forgot how much eosin and what %
to put in the last 100% of the Tissue Processor to show-up small transparent
bx's. Can one of you wonderful people tell me please. The processor I am
using
is a VIP 5.
Thanks a Bunch!
Ak
Happy Friday Everyone,
I guess I am having a old persons momment! I forgot how much eosin and what %
to put in the last 100% of the Tissue Processor to show-up small transparent
bx's. Can one of you wonderful people tell me please. I tissue processor is a
VIP 5.
Thanks a bunch!
Akemi Alliso
Happy Friday Everyone,
I am sending this inquiry again. For some reason this post did not
show I my histonet emaiI. I guess I am having a old persons
momment! I forgot how much eosin and what % to put in the last 100%
of the Tissue Processor to show-up small transparent bx's. Can one
I know using eosin as a tissue dye during processing has been a recent
discussion. My lab has been experiencing issue with the cobalt blue we
use currently. At embedding, small biopsies are not seen on blue
sponges, white sponges turn blue. Biopsies that are wrapped are not
turning blue, the pape
:
> Does anyone use eosin in their Peloris?
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's
> Sent: Friday, October 22, 2010 2:07 PM
> To: histonet@lists.utsouthwestern.ed
Does anyone use eosin in their Peloris?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's
Sent: Friday, October 22, 2010 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eos
This is the best kept secretFor those who have never tried this, you don't
know what you are missing in the ease of embedding and cutting.
We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100%
alcohols, leaving the last 100% pure (of course there is carry over during th
...@imagesbyhopper.com
Sent: Friday, October 22, 2010 8:18 AM
To: Tench, Bill
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin
It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol!
I agree with Bill, it really makes a HUGE difference in small, minute
@lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin
It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol!
I agree with Bill, it really makes a HUGE difference in small, minute tissues.
We did run into one issue though, we were using orange biopsy cassettes and the
red
It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol!
I agree with Bill, it really makes a HUGE difference in small, minute tissues.
We did run into one issue though, we were using orange biopsy cassettes and the
red-orange tissue was difficult to spot in the
I think the use in a processor becomes a local culture. I think
everyone in my community does it (someone spread the word), so now we
all do. I believe that we are all putting it in the last alcohol. It
really makes facing a block on minute pieces a whole lot easier. We
found marking the tissue
. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
"Scott, Allison D"
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/21/2010 04:37 PM
To
cc
Subject
[Histonet] Eosin to dye small Biopsies
Hello to all in histoland. Are any of you using eosin on the proces
We use cobalt blue as it does not interfere with FISH
Sent from my Verizon Wireless Phone
- Reply message -
From: susan.wal...@hcahealthcare.com
Date: Fri, Oct 22, 2010 3:28 am
Subject: [Histonet] Eosin to dye small Biopsies
To: ,
Cc:
We use about the same amount but in our last 100
:
Subject: Re: [Histonet] Eosin to dye small Biopsies
We use about 40ml of eosin in the first 100% alcohol in both of our "large
specimen" & "small biopsy" machines.
Michelle
On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"
wrote:
> Hello to all in histolan
@lists.utsouthwestern.edu
Subject: [Histonet] Eosin to dye small Biopsies
Hello to all in histoland. Are any of you using eosin on the processor
to dye your small bx's? If so, are you putting it in the 100% alcohol
to do so? Any help in this matter will be greatly appreciated.
Allison Scott HT
We use about 40ml of eosin in the first 100% alcohol in both of our "large
specimen" & "small biopsy" machines.
Michelle
On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"
wrote:
> Hello to all in histoland. Are any of you using eosin on the processor
> to dye your small bx's? If so, are you
Hello to all in histoland. Are any of you using eosin on the processor
to dye your small bx's? If so, are you putting it in the 100% alcohol
to do so? Any help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
CONFIDENTIAL
@lists.utsouthwestern.edu; sgoe...@xbiotech.com
Inviato: Lun 18 ottobre 2010, 17:51:43
Oggetto: Re: [Histonet] Eosin
Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if
you
observe a solution with transmitted light.
René J.
--- On Mon, 10/18/10, sgoe...@xbiotech.com wrote:
From: sgoe
Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if
you observe a solution with transmitted light.
René J.
--- On Mon, 10/18/10, sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com
Subject: [Histonet] Eosin
To: histonet@lists.utsouthwestern.edu
Date: Monday
Once upon a time I heard that eosin fluoresces is this true? = ; What
color does it show up? Could this be used as a sort of back gr= ound
stain so you can tell exactly how many cells are in the field?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
= = XBiotech USA Inc.
8201
There shouldn't be any problem if you are doing immunoperoxidase staining. If
you are doing fluorescence, you will have to be sure you remove all traces of
eosin from the tissues since eosin itself is fluorescent.
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Histonet@li
few choices available to you. If
you need some suggestions drop me an email off list.
Have a nice weekend,
Amos
Message: 7
Date: Fri, 21 May 2010 14:06:40 -0700
From: sgoe...@xbiotech.com
Subject: [Histonet] Eosin in Processor and iHC
To: histonet@lists.utsouthwestern.edu
Message-ID
Has anyone had any trouble prestaining tissues in eosin and then
doing IHC? I'm making cell blocks from clear fluid full of cells
embe= dded in agar before processing and I just was wanting a way to
be able to s= ee it. Does anyone see a problem with a drop of eosin
;
To: histonet@lists.utsouthwestern.edu
Sent: Tue, March 9, 2010 7:51:46 AM
Subject: [Histonet] Eosin too pink
Our Pathologists have, over the last few weeks, complained that the eosin is
too pink. We have not altered our procedures or reagents in any way. It seems
to be particularly bad in endo
Our Pathologists have, over the last few weeks, complained that the eosin is
too pink. We have not altered our procedures or reagents in any way. It seems
to be particularly bad in endoscopic biopsies. Thanks
Lesley Smith
Senior Biomedical Scientist Cellular Pathology
The Yorkshire Clinic
Bradf
Where do you get the beads from?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Joyce
Cline
Sent: Wednesday, February 03, 2010 2:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin
February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an H&E stained slide?
Thanks,
Scott Hendricksen HT(
...@lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an H&E sta
Your xylene and/or alcohols may need changing.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen
Sent: Wed 2/3/2010 9:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
>
> Hi again,
>
> Does anyone have an issue with eosin leaching out of sections a few days
> after they have been coverslipped on an H&E stained slide?
>
> Thanks,
>
> Scott Hendricksen HT(ASCP)
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Hi again,
Does anyone have an issue with eosin leaching out of sections a few days after
they have been coverslipped on an H&E stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
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http://lists.
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days after
they have been coverslipped on an H&E stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
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Before going home, cover the container
René J.
--- On Fri, 1/15/10, Cheri Miller wrote:
From: Cheri Miller
Subject: [Histonet] Eosin evaporation.
To: "histonet" ,
"histonet-boun...@lists..utsouthwestern.edu"
Date: Friday, January 15, 2010, 10:05 AM
I recent
Behalf Of Cheri
Miller
Sent: Friday, January 15, 2010 10:06 AM
To: histonet; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Eosin evaporation.
I recently purchased a leica refurbished autostainer, Before we hand stained
our by hand. Now I notice the eosin is getting very low very
During the winter months the air is drier, especially with central heat.
My tap water is sometimes as if it has been refrigerated too.
Paula
From: Sheila Haas
To: Cheri Miller ; "Sherwood, Margaret"
; "Breeden, Sara" ; "Mahoney,
Janice A" ; histonet
;
"h
I recently purchased a leica refurbished autostainer, Before we hand stained
our by hand. Now I notice the eosin is getting very low very fast, we have to
replenish almost everyday. Before a bottle of Richard Allen Eosin would last
the week or at least six days. Any idea's?
Cheryl Miller HT ASC
ldn't be surprised
if it interfered with eosin binding.
> --
> From: histonet-boun...@lists.utsouthwestern.edu on behalf of Goins,
> Tresa
> Sent: Tuesday, August 4, 2009 5:11 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet]
We very occasionally have a single slide out of a group of 100 or more where
the eosin will bleed after coverslipping. Does anyone know if this is
associated with a particular tissue type or treatment with Nair to soften
keratin?
Thanks,
Tresa
Veterinary Diagnostic Lab
Bozeman, MT
___
om McNemar
Sent: Wednesday, July 15, 2009 3:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin in Alcohol
News to me We have used this for many years and have never had a problem
with IHC ourselves or heard of anyone else having issues.
Tom McNemar, HT(ASCP)
Histology Co
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Harrison,
Sandra C.
Sent: Tuesday, July 14, 2009 5:15 PM
To: Jennifer Johnson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin in Alcohol
"polyc
day.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Johnson
Sent: Tuesday, July 14, 2009 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin in Alcohol
A couple of weeks ago I p
A couple of weeks ago I posted the message below on the histonet and all of you
responded that it shouldn't matter so I have finally gotten a reply from the
company we send our prostate biopsies off to and below is their response. So
now you know the rest of the story!
We have used Eosin i
we put phloxine in the formalin - we find it a better option than eosin
2009/7/1 Beth Couch
> Hi Histonetters - do any of you use easin in the processing alcohols in
> order to better visualize small bipsies and if so how much eosin to alcohol
> do you use and in which alcohol?
> Thank you in a
Hi Histonetters - do any of you use easin in the processing alcohols in
order to better visualize small bipsies and if so how much eosin to alcohol
do you use and in which alcohol?
Thank you in advance for your help.
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The eosin should be acidic and you get to that point adding 1% aq. sol. of
acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin
solution.
René J.
--- On Mon, 10/20/08, Hana Peter <[EMAIL PROTECTED]> wrote:
From: Hana Peter <[EMAIL PROTECTED]>
Subject: [His
Hi!
Can someone tell me the right pH for eosin y 1% aqueous solution?
The one I made from powder has pH 6.3. Is this OK? The commercial one we
usually buy is a bit lower (5.7).
Thank you in advance!
Hana Peter
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