Hi Histonetters, Would anyone be willing to share how they process a Fat Pad aspirate for Amyloidosis? Our method does not provide a very good result and we're looking to improve on how we do this.
We collect direct aspirates using an 18 gauge needle and make air dried smears and stain with Congo Red. We also get additional passes and place directly in 10% NBF and will try to make a cell block using histogel out of them. Problem is fat floats and it is difficult to get a good cell block made. I would think spinning the 10%NBF and filtering out the fat and placing directly into a biopsy bag or wrapping in paper would be the way to go. After you process the block, I'm guessing all the lipid will process out and there would only be cell membranes from the fibroadipose tissue left over. Wouldn't you need a significant amount of fat to process to have anything to embed? I've seen suggestions on other websites suggesting 35-50 mg of fat. Maybe we need to go to a larger needle, maybe 16 gauge? Any ideas would be greatly appreciated! Scott Scott A. Lindrud, MLS(ASCP) CM CT CM Histopathology Technical Specialist/Cytotechnologist CarrisHealth Rice Memorial Hospital 301 Becker Ave SW | Willmar | MN | 56201 scott.lind...@carrishealth.com 320-231-4406/4520 320-231-4503 (fax) Carris Health is a new entity created to deliver health care to West Central and Southwest Minnesota. Carris Health is a partnership between CentraCare Health, Rice Memorial Hospital and ACMC Health. www.carrishealth.com<http://www.carrishealth.com/> Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Health Information Systems Network Security staff by calling the IS Help Desk for assistance at (320) 251-2700 ext. 54540 and delete these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
