Use Bouin's fixative.
René J.
--- On Mon, 8/10/09, Cheryl Crowder wrote:
From: Cheryl Crowder
Subject: [Histonet] Fixation of embryos
To: "Histonet"
Date: Monday, August 10, 2009, 11:27 AM
Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.
After proc
If the G in 4F1G is glutaraldehyde the glut. will probably kill off the
immunoreactivity (depending on the antigen in question).
How long are the embryos being fixed? Remember, formalin works slowly so
5-7 days may be necessary.-
I would try a formalin + alcohol mix or formalin-alcohol-acetic ac
Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.
After processing they are mushy and almost amorphous. Has anyone any
suggestions for a fixative that will give the embryos more form and still
have the tissue capable of doing IHC? I had thought of 4F1G. Any help is
re
