Thanks, Dont you think high pressure during perfusion leads to tissue swelling
and disrupted morphology?
2009-01-13
TF
发件人： Charles Scouten
发送时间： 2009-01-13 00:02:40
收件人： ti...@foxmail.com
抄送： histonet
主题： RE: Re: [Histonet] Fixation of the damaged brain tissue
Formaldehyde
kull into PFA for 1-2 days.
It should work...
The perfusion is not well in many conditions - in my injury model, part of the
blood supply was interrupted.
2009-01-12
TF
发件人： Geoff McAuliffe
发送时间： 2009-01-12 22:59:22
收件人： tifei
抄送： histonet
主题： Re: [Histonet] Fixation of the damaged b
Hi TF:
Your problem should not be happening.
Sounds like poor perfusion followed by immersing the whole brain (with
the wound deep in the interior) in a small volume of fix at 4 degrees C.
What fixative are you using?
Geoff
TF wrote:
Hi, I just want to fix the damaged brain tissue (2-48 hour
Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the
injury).
Because the injury damaged the surrounding blood supply, and after perfusion I
normally got a mass of soft, shapeless tissue surrounding the injury site...
I have also tried post-fixation of 24-48 hours before r
