I stand correct on the perfusion under anesthesia issue. Jerry Ricks Research Scientist University of Washington Department of Pathology
Date: Tue, 30 Jun 2009 12:37:20 -0700 Subject: Re: [Histonet] Good Perfusion... From: chana.de.w...@gmail.com To: jf...@gladstone.ucsf.edu CC: rosenfeld...@hotmail.com; histonet@lists.utsouthwestern.edu Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish <jf...@gladstone.ucsf.edu> wrote: Dear Jerry and histonetters, I don't believe this is unethical or illegal. It is written into our IACUC approved protocols as follows: Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin or Halothane induced. Bilateral thoracotomy." Could it only be forbidden by your facility? I wonder if other facilities have rules against such things. But we insist that our investigators use perfusion under anesthsia because the deeper tissues are much better fixed and blood removal is more thorough and complete by this method. Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jf...@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Tuesday, June 30, 2009 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Perfusion... No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Insert movie times and more without leaving HotmailR. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Insert movie times and more without leaving HotmailĀ®. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet