Re: [Histonet] H&E Staining question

2019-02-27 Thread Jay Lundgren via Histonet
Obviously, you're going to get a much different result using a polychromatic stain, than by doing a standard H&E. But if the pathologists like it... I'm not even sure if they could call that stain an H&E in their dictation. Seems like that would open them up to liability if, God forbid, they ever

[Histonet] H&E Staining question

2019-02-27 Thread Charles Riley via Histonet
Has anyone tried using Hologic's Thin Prep solutions (Nuclear stain, rinse, and bluing) to do their H&E stains? I am trying to validate a new stainer and in the process the pathologists want to tweak our protocol to get a better stain and thought maybe we could try this to see about saving money i

Re: [Histonet] H&E staining question

2013-07-02 Thread Colleen Forster
I am interested in the answer as I use this same technique on my rodent samples and have not had this issue. On 7/2/2013 8:07 AM, Connolly, Brett M wro Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues coul

[Histonet] H&E staining question

2013-07-02 Thread Connolly, Brett M
Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks