Hi Cassie,
I would strongly suspect your tap water. If you have a tap water rinse before
hematoxylin, the pH or treatment additives carrying over could be weakening
your stain. We fixed this problem previously by adding a bucket of DI water
between the tap water wash and the hematoxylin, and th
uary 23, 2016 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H & E Troubleshooting
Hi Histo folks,
We are having a fun time troubleshooting our H&E because the problem
in not consistent...
Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcoh
Watch your soap residue in the staining dishes. That will mess you up.
Bleach does leave a residue, that¹s why we then wash in soap.
But if you wash in soap you must use a soap residue measurement to prove
the soap is rinsed clean.
It will change the pH of your Heme. Spent a long 6 months figuring
Hi Histo folks,
We are having a fun time troubleshooting our H&E because the problem
in not consistent...
Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcohol
since our ordered had not arrived yet. One of our doctor said our hematoxylin &
eosin was readable but
