[Histonet] Help cutting horse tendon

2010-11-25 Thread Alex Cummins
I am new to the list so I don't know your experience. If your spaces are too wide did you cryoprotect before freezing? As freezing when done right gives very consistent size retention. Alex ___ Histonet mailing list Histonet@lists.utsouthwestern.edu ht

[Histonet] Help cutting horse tendon.

2010-11-24 Thread Pablo Sanchez-Quinteiro
Dear listers, I am trying to cut whole sections of the tendon of the digital flexor muscle of the horse. The piece is around 1 mm long and very elastic and fibrous. I have tried formalin and Bouin fixation, the latest better. With paraffin embedding the fibrous elements of the tendon spoil th