either,
has anyone figured this out?
Esther
From: histonet-boun...@lists.utsouthwestern.edu
on behalf of John Kiernan
Sent: Tuesday, January 21, 2014 8:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histogel
The document at
http://lists.utsouthwestern.edu/mailman/li
dusko trajkovic
> Sent: Monday, January 20, 2014 12:47 PM
> To: Esther C Peters; jennifer.arcand-john...@genzyme.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histogel
>
> Esther,
> I mainly process cells, which have been spun down into a small pellet. Also
> mou
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histogel
Esther,
I mainly process cells, which have been spun down into a small pellet. Also
mouse DRG's and other very small tissues. I would consider this delicate, so do
not be afraid to use a longer processing program. Histogel/
her C Peters
To: "jennifer.arcand-john...@genzyme.com"
; "histonet@lists.utsouthwestern.edu"
; dusko trajkovic
Sent: Monday, January 20, 2014 11:15 AM
Subject: RE: [Histonet] Histogel
Thank you, Dusko!
I have had the same problem with 1.5% agarose, and I tried starting
ehalf of dusko trajkovic
Sent: Monday, January 20, 2014 1:58 PM
To: jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histogel
Jennifer,
You might have seen one of my posts from 2-3 years ago. I had the exact same
problems you described. Could not get anyone t
. Longer
processing is the answer, and nothing else.
Good Luck.
Dusko Trajkovic
Pfizer Inc. La Jolla
858-638-6202
From: "jennifer.arcand-john...@genzyme.com"
To: histonet@lists.utsouthwestern.edu
Sent: Monday, January 20, 2014 7:56 AM
Subject: [Histonet
Dear Histonetters,
I have been reading up on the archives for info on Histogel. Previous posts
discuss how they had problems with it - some samples would come out great and
some would shrivel up or even dissolve.
These posts on the Histogel were from a few years ago and was hoping, and
praying
ocess it as I would regular tissue. Cuts very well
too.
Katy
Message: 3
Date: Fri, 24 Jun 2011 12:26:46 -0400
From: "Dessoye, Michael J"
Subject: [Histonet] HistoGel
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hello,
Does anyone out th
me know if you need any further info or explanation.
Dusko Trajkovic HT ASCP
Pfizer Inc. La Jolla
From: Amos Brooks
To: histonet@lists.utsouthwestern.edu
Sent: Fri, June 24, 2011 10:29:09 AM
Subject: [Histonet] Histogel Problem
Hi,
I have a problem with
n.edu
Subject: [Histonet] Histogel Problem
Hi,
I have a problem with some blocks that were prepared with Histogel. I was
hoping someone else might have had a similar problem and figured it out. I
took a photo of the blocks that were mads and put them in a Picassa album
here:
https://picasaweb.googl
Hi,
I have a problem with some blocks that were prepared with Histogel. I was
hoping someone else might have had a similar problem and figured it out. I
took a photo of the blocks that were mads and put them in a Picassa album
here:
https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3
Hello,
Does anyone out there have any experience with HistoGel? It's Richard
Allan/Thermo Fisher. They claim that you can "embed" scant tissues in the gel
and then process, embed, and cut as usual. Just wondering how it works in the
real world
Michael J. Dessoye, M.S. | Histology Supe
A little bit of plain agar, or a plain TSA slant tube (stolen from
micro), melted in the microwave for <10 seconds, works just as well as
histogel, imho.
Jay A.
Lundgren M.S., HTL (ASCP)
One of my pathologists is interested in the pros and cons of HistoGel for cell
blocks. I have read the archives and gathered some information for him. He
specifically would like to talk to someone that has been using it for some time.
Along the same line, what method do you find to be the best
Next day, pellet cells and resuspend them in a small amount (100-200
microliters) of Histogel or Agar. When the gel cools cut the microcentrifuge
tube open with a scalpel or razor blade. Now you have a nice cell pellet
shaped like the bottom of your centrifuge tube, and you can treat it just l
Hello everyone! If you are using Histogel for your cytology cell blocks, how
do you liquify it? Do you use an incubator or microwave? How happy are the
pathologists with the quality of the cell blocks?
Thanks in advance for your information.
Carol Bryant, CT (ASCP)
Cytology/Histology Manager
> histogrl backlash
thats a whole 'nother set of issues altogether ;)
happy friday!
anh2...@med.cornell.edu wrote:
Wow, what is the histogrl backlash? Will someone please summarize the issues
people are having?
Thanks!
Andrea
-Original Message-
From: "Hofecker, Jennifer L"
Date:
Wow, what is the histogrl backlash? Will someone please summarize the issues
people are having?
Thanks!
Andrea
-Original Message-
From: "Hofecker, Jennifer L"
Date: Fri, 20 Mar 2009 08:23:02
To: ; Andrea Grantham
Subject: RE: [Histonet] processing v-e-r-y tiny samples
Hi Andi, Happy
Has anyone tried embedding a block of tissue like a mouse brain in Histogel,
and then cutting frozen or on a vibratome?
I am doing Golgi stain on mouse brain, and the tissue is VERY friable.
Website says, "No matter how small, friable, or viscous your histology
or cytology specimen, HistoGel will
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